食管鳞癌中血管内皮生长因子－C 的表达及与淋巴管生成的关系

摘要

Objective:To study the expression of vascular endothelial growth factor -C(VEGF -C)in esophageal squamous cell carcinoma(ESCC)and its relation with lymphangiogenesis and lymphatic metastasis.Methods:Collect 107 cases of ESCC resection and 56 paraffin -embedded tissues of normal esophageal tissues and by immunohisto-chemical detection to examine the expression of VEGF -C in ESCC.To observe micro -vascular endothelial cells in tagged tissues to take count of the micro -lymphatic vessel density(LVD).Results:The high rate of expression of VEGF -C protein in the ESCC was 58.9%,which was significantly higher than that of normal esophageal tissue and this difference has statistical significance(P <0.05).The high rate of expression of VEGF -C protein in the group of lymphatic metastasis was 68.3%,while that of VEGF -C protein in the group without lymphatic metastasis was 45.5%(P <0.05).The high rate of expression of VEGF -C protein in the group of T3 /T4 was 65.7%,while that of VEGF -C protein in the group of T1 /T2 was 45.9%(P <0.05).But there was no relativity between the high rate of expression of VEGF -C protein and ESCC patient's age,gender,degree of differentiation and tumor size(P >0.05), among the high rate of expression of VEGF -C protein in different TNMphases of ESCC.The LVD of ESCC in differ-ent expression intensity groups of VEGF -C in each group had statistical significance(P <0.05).Conclusion:VEGF-C gene may promote the lymphangiongenesis and the lymphatic metastasis of ESCC,and may become a laboratory index of predicting the prognosis of ESCC.%目的：探讨血管内皮生长因子－C（VEGF －C）在食管鳞癌中的表达及其与淋巴管生成、淋巴结转移的关系。方法：收集2013年3月至2014年1月遂宁市中心医院胸心外科的107例食管鳞癌手术切除病例及56例正常食管组织的石蜡包埋组织样本，采用免疫组化检测 VEGF －C 在食管鳞癌中的表达，并应用 D2－40抗体标记组织中的微淋巴管内皮细胞，计数微淋巴管密度（LVD）。同时对其与临床病理参数的关系进行分析。结果：食管鳞癌中 VEGF －C 蛋白的高表达率明显高于正常食管组织，且在食管鳞癌不同 TNM分期中有差异（P ＜0．05）；淋巴结转移组中 VEGF －C 蛋白的高表达率为68．3％，高于无淋巴结转移组（P ＜0．05）。T3／T4期组中 VEGF －C 蛋白的高表达率高于 T1／T2期组（P ＜0．05）。VEGF －C 蛋白的高表达率与食管鳞癌患者的年龄、性别、分化程度、肿瘤大小等均无明显相关性（P ＞0．05）。食管鳞癌中 LVD 在 VEGF －C 不同表达强度组中有差异，差异有统计学意义（P ＜0．05）。食管鳞癌淋巴结转移组 LVD 高于无淋巴结转移组，差异均有统计学意义（P ＜0．05）。结论：VEGF －C 基因可能促进食管鳞癌的淋巴管生成及淋巴结转移，有可能成为预测食管鳞癌预后的实验室指标。