食管鳞癌中肝细胞生长因子、血管内皮生长因子C及其受体3的表达及意义

摘要

Objective:To investigate the role of HGF,VEGF-C,VEGFR-3 on the occurrence and development of human esophageal squamous cell carcinoma.Methods:RT-PCR technique was used to examine expression of HGF,VEGF-C and VEGFR-3 mRNA and immunohistochemical staining was used to examine expression of HGF, VEGF-C and VEGFR-3 in 25 cases of normal mucosa and 60 cases of ESCC tissues.Results:Expression of HGF,VEGF-C and VEGFR-3 in ESCC showed significantly higher than those in normal mucosa (P< 0.01,P< 0.01,P< 0.01),and they were positively correlated with lymph node metastasis and TNM stage of ESCC.Conclusion:The high expression of HGF,VEGF-C and VEGFR-3 may play an important role in the occurrence and development of ESCC.HGF,VEGF-C and VEGFR-3 may interact with each other to promote the generation and development of ESCC.%目的:探讨肝细胞生长因子(HGF)、血管内皮细胞生长因子C(VEGF-C)和其受体3(VEGFR-3)在食管鳞癌中的表达水平及与食管鳞癌临床病理因素之间的关系.方法:采用RT-PCR技术检测25例食管正常黏膜、60例食管鳞癌组织中HGF、VEGF-C、VEGFR-3 mRNA的表达水平,并应用免疫组织化学法检测HGF、VEGF-C、VEGFR-3蛋白的表达水平并分析其与食管鳞癌临床病理因素之间的关系.结果:食管鳞癌组织中HGF、VEGF-C、VEGFR-3表达量明显高于食管正常黏膜组织,并且HGF、VEGF-C、VEGFR-3与食管鳞癌淋巴结转移、TNM分期呈正相关.结论:HGF、VEGF-C、VEGFR-3在食管鳞癌的发生发展中起到重要作用,三者可能协同作用在食管癌浸润生长、淋巴结转移及远处播散过程中起到一定作用.