番茄红素对肺癌A549细胞增殖和凋亡的影响及其机制研究

摘要

Objective:To investigate the effects and mechanism of lycopene (LP) on the proliferation and apoptosis of human non-small cell lung cancer A549 cell.Methods:The human non-small cell lung cancer A549 cells in logarithmic growth phase were treated with lycopene (2.5,5,10,20 μg/ml) and cisplatin (40 μg/ml).The cellular growth inhibition rate was tested by MTT.Cell cycle and apoptosis rates were analyzed by flow cytometry (FCM).The expression of apoptosis-related genes (Bax mRNA and bcl-2 mRNA) were detected by RT-PCR,and the expression of caspase-3 protein was detected by Western blot.Results:Compared with blank control group,the cellular growth inhibition rate of human non-small cell lung cancer A549 cell in LP treated groups were significantly increased.The cell cycle was arrested at G0/G1 phase,the apoptosis rate was significantly increased.The expression of Bax mRNA was significantly increased,while the expression of bcl-2 mRNA was significantly decreased,and the ratio of Bax/bcl-2 was significantly increased.The expression of caspase-3 protein was significantly increased.All of the effects of lycopene above were dose-dependent with a certain.Conclusion:LP can inhibit human HepG2 cell proliferation and promote its apoptosis,which perhaps related to its effects of altering the cell cycle and the expression of apoptosis-related genes and proteins.%目的:研究番茄红素(lycopene,LP)对人非小细胞肺癌A549细胞增殖和凋亡的影响并探讨其机制.方法:体外培养并取对数生长期人非小细胞肺癌A549细胞,分别给予不同浓度的LP(2.5、5、10、20 μg/ml)和顺铂(40 μg/ml)进行干预,48 h后采用四甲基偶氮唑盐(MTT)比色法测定吸光度值并计算细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和细胞凋亡状况,逆转录PCR法(RT-PCR)检测细胞中凋亡相关基因(BaxmRNA、bcl-2 mRNA)表达,免疫印迹法(Western blot)检测凋亡相关蛋白(caspase-3)表达.结果:与空白对照组比较,LP能够剂量依赖性地提高人非小细胞肺癌A549细胞增殖抑制率,延长细胞周期中G0/G1期并缩短G2/M期,提高A549细胞凋亡率(AI),上调Bax mRNA表达、下调bcl-2 mRNA表达,提高Bax/bcl-2比值,上调caspase-3蛋白表达.结论:LP具有抑制人非小细胞肺癌A549细胞增殖并促进其凋亡的作用,其机制可能与LP能够阻滞细胞周期并调节凋亡相关基因蛋白表达有关.