Survivin及P-糖蛋白在乳腺癌组织中的表达及意义

摘要

Objective:To explore the expression and clinical significance of anti-apoptotic Survivin protein and multidrug resistance proteins P-glycoprotein in human breast cancer tissues.Methods:Survivin and P-glycoprotein were detected by immunohistochemistry with tissue microarrays which contained 217 breast cancers and 52 cases of mammary gland fibroma.Comparative the differences of Survivin and P-glycoprotein,and the correlation of expression of Survivin and P-glycoprotein with clinicopathologic markers (including:the pathological type,tumor size,lymph node metastasis,TNM stage,histological grade,etc.) of breast cancer were analyzed.Results:Positive rates of Survivin and P-glycoprotein in mammary gland fibroma were 13.46％ and 9.62％,significantly lower than 76.04％ and 73.27％ positive rates in breast cancers respectively(P ＜ 0.05).Correlation analysis showed there was a weak positive correlation between Survivin and P-glycoprotein (P ＜ 0.05).Survivin had positive correlation with lymph node metastasis,TNM stage and histological grade (P ＜ 0.05).P-glycoprotein had positive correlation with histological grade(P ＜0.05).The difference of mean survival time in positive Survivin,positive P-glycoprotein and negative Survivin protein,negative P-glycoprotein were respectively statistical significance (P ＜ 0.05).Conclusion:There were high-level expression of Survivin and P-glycoprotein in breast cancer tissues,both Survivin and P-glycoprotein had positive correlation with histological grade,affected survival time of patients.Survivin had a weak positive correlation with P-glycoprotein.%目的:探讨抗凋亡蛋白Survivin及多药耐药的P-糖蛋白在乳腺癌组织中的表达及其意义.方法:应用免疫组化方法检测217例乳腺癌及52例乳腺纤维瘤中Survivin蛋白和p-糖蛋白的表达,比较Survivin 蛋白和p-糖蛋白表达的差异、分析乳腺癌临床病理学指标(包括:病理类型、肿瘤大小、淋巴结转移、TNM分期、组织学分级等)与Survivin蛋白和p-糖蛋白表达的关系,并进行生存分析.结果:乳腺纤维腺瘤中Survivin蛋白和p-糖蛋白的阳性率分别为13.46％和9.62％,乳腺癌中Survivin蛋白和p-糖蛋白的阳性表达率分别为76.04％和73.27％,差异均有统计学意义(P＜0.05);相关性分析表明:Survivin抑凋亡蛋白及p-糖蛋白表达具有弱正相关性(P＜0.05).Survivin与TNM分期、淋巴结转移、组织学分级正相关;p-糖蛋白与组织学分级正相关(P＜0.05).Survivin蛋白阳性与p-糖蛋白阳性患者的平均生存时间分别与Survivin 蛋白阴性、p-糖蛋白阴性的差异具有统计学意义(P＜0.05).结论:Survivin蛋白与P-糖蛋白在乳腺癌组织中呈现高表达,且两者表达均与组织学分级正相关,影响患者生存时间,Survivin蛋白及p-糖蛋白表达具有弱正相关性.