高强度聚焦超声对体外胰腺癌细胞凋亡和STAT3/pSTAT3蛋白表达的影响

摘要

Objective:To explore the high-intensity focused ultrasound( HIFU)in treatment of in vitro PANC-1 pancreatic cancer cell lines,then observe still alive cells of STAT3/pSTAT3 protein expression and the change of cell apoptosis. Methods:To establish in vitro agarose gel model embedded human pancreatic tissue,then were randomly divided into control and experimental group and tagged. Experimental group was treated by type JC200 HIFU thera-peutic equipment,control group without any treatment. The specimen pathological changes were observed by HE dye-ing and the STAT3/pSTAT3 protein expression changes were observed by immunohistochemistry. In addition,in vitro experimental model of pancreatic cancer cell lines were respectively irradiated with different doses of HIFU( 0J, 100J,200J,400J,800J). After that,observing pancreatic cancer cell proliferation inhibition by MTT,and detecting STAT3/pSTAT3 expression by Western blot. Results:After HIFU treatment,in vitro of pancreatic cancer target tissues histopathological obviously observed solidified necrosis,and cell structure disappeared. Immunohistochemical staining observed target tissues brown granules decreased significantly,which demonstrated STAT3/pSTAT3 protein expression decreased after treatment. MTT cell proliferation rate demonstrated control group(0J)compared to low-power groups (100J,200J,400J)had no significant differences in cell proliferation,and high-power group(800J)significantly reduced the rate of cell proliferation,no significant difference between groups(P﹥0. 05). Western blot tested expres-sion of STAT3/pSTAT3 suggested low-power groups(100J,200J,400J)had no significant differences with the con-trol group. Conclusion:HIFU can effectively treat pancreatic cancer,which may be related to destroy tumor cells themselves structure and STAT3/pSTAT3 protein expression.%目的：探讨高强度聚焦超声（ high-intensity focused ultrasound，HIFU）体外处理胰腺癌细胞株PANC-1后仍存活细胞的STAT3/pSTAT3蛋白表达和细胞凋亡的变化及关系。方法：建立琼脂凝胶包埋的人胰腺癌组织体外实验模型，随机分为对照组和实验组并标记，实验组行JC200型HIFU治疗仪治疗，对照组不做任何处理，HE染色观察标本病理变化；免疫组化观察STAT3/pSTAT3蛋白表达变化。再将体外胰腺癌细胞实验模型分别行不同剂量的HIFU照射（0J，100J，200J，400J，800J），通过MTT观察胰腺癌细胞的增殖抑制情况，通过Western blot检测STAT3/pSTAT3表达情况。结果：经HIFU治疗后，离体的胰腺癌靶区组织病理可以看到明显的凝固型坏死灶，细胞结构消失，免疫组化染色可见HIFU治疗的靶区组织棕色颗粒明显减少，提示治疗后STAT3/pSTAT3蛋白表达减低；MTT细胞增殖率检测提示与对照组（0J）相比，低功率组（100J，200J，400J）细胞增殖抑制无明显差异，高功率组（800J）的细胞增殖率明显减低，组间比较差异无统计学意义（ P﹥0.05）。Western blot检测STAT3/pSTAT3表达提示低功率组（100J，200J，400J）与对照组无明显差异。结论：HIFU能有效的治疗胰腺癌，其机制可能与破坏肿瘤细胞自身结构及影响STAT3/pSTAT3蛋白表达有密切关系。