TNF相关的凋亡诱导配体和受体在不同胸腺组织中的差异表达及对细胞凋亡的影响

摘要

Objective:To investigate the differential expression of TNF-related apoptosis-inducing ligands and receptors in different thymus tissues,and provide a reference for a role of TRAIL in thymus biology and disease treatment.Methods:6 patients with myasthenia gravis associated with thymic hyperplasia,14 patients with myasthenia gravis associated with thymoma,and 10 normal subjects were selected as the subjects.The differential expression profiles of TNF-related apoptosis-inducing ligands and its transmembrane receptors,nuclear factor-κB activation status,and apoptotic cell counts in normal thymus,thymic hyperplasia and thymoma were investigated.Results:TNF-related apoptosis-inducing ligands and its receptors were expressed in all thymus tissues.The expressions of DR4 and DR5 in thymic hyperplasia were the highest.The active forms of NFκB in all thymus tissues were not detected.Compared with normal thymus,the apoptotic cell counts in thymic hyperplasia and thymoma tissue were higher.Conclusion:The expression levels of TRAIL death receptor(DR4/DR5) among the normal thymus,thymic hyperplasia and thymoma were significantly different.And the TRAIL has important potential value in selective induction of apoptosis in thymus tumor cell.%目的:研究肿瘤坏死因子(TNF)相关的凋亡诱导配体(TRAIL)和受体在不同胸腺组织中的差异表达,为TRAIL在胸腺生物学及疾病治疗中的作用提供参考依据.方法:选择6例伴有胸腺增生和14例伴有胸腺瘤的重症肌无力患者以及10名正常受试者作为研究对象,分别分析正常胸腺、胸腺增生和胸腺瘤组织中的TRAIL及其跨膜受体的差异表达谱、NFκB活化状态和凋亡细胞指数.结果:所有胸腺组织均可表达TRAIL及其受体,胸腺增生组织的死亡受体DR4和DR5的表达水平最高.所有胸腺组织中NFκB的活性形式均未检测到.与正常胸腺相比,胸腺增生和胸腺瘤组织中的凋亡细胞指数更高.结论:TRAIL死亡受体(DR4/DR5)在正常胸腺、胸腺增生和胸腺瘤组织中的表达水平呈现显著差异,TRAIL在选择性诱导胸腺肿瘤细胞凋亡方面具有重要的潜在价值.