肿瘤干细胞标志物在鼻咽癌细胞株中的表达

摘要

Objective To explore the expression of cancer stem cells(CSC) markers named ABCG2,CD44,CD34 in hu man nasopharyngeal carcinoma(NPC) CNE-2,5-8F and 6-10B cell lines to provide the basis of CSC markers research of NPC. Methods The NPC cell lines of CNE2,5-8F and 6-10B were cultivated regularly,of which,ABCG2,CD44,CD34 cell ratio was detected with flow cytometry. Results ABCG2 positive cells accounted about 0.1%,18.6%and 19.9%in the CNE-2,5-8F,6-10B cell lines. CD44 positive cells accounted 99.5%,93.2%and 99.1%in the CNE-2,5-8F and 6-10B cell lines. CD34 positive cells accounted 0,3.0%and 0.1%in the CNE2,5-8F,and 6-10B cell lines. ABCG2+CD44+cell interation accounted about 11.6%and ABCG2+CD44+cell interation accounted about 0 in 5-8F cell lines. Conclusion The expression proportion of CD34+in the 5-8F and 6-10B cell lines confirms to CSC markers of the CSC theory. CD34 may be deemed as a candidate marker of nasopharyn-geal neoplas.%目的探索肿瘤干细胞（CSC）标志物ABCG2、CD44、CD34在鼻咽癌细胞株CNE2、5-8F和6-10B中的表达情况，为选择CSC标志物进行鼻咽癌CSC样研究奠定基础。方法常规培养CNE2、5-8F和6-10B这3种鼻咽癌细胞株，用流式细胞仪检测3种鼻咽癌细胞株中ABCG2、CD44、CD34细胞比例及其交互情况。结果 ABCG2在CNE2、5-8F和6-10B这3种鼻咽癌细胞株中表达率分别为0.1％、18.6％、19.9％；CD44在CNE2、5-8F和6-10B中表达率分别为99.5％、93.2％、99.1％；CD34在CNE2、5-8F和6-10B 中表达率分别为0、3.0％、0.1％。在5-8F 中，ABCG2+CD44+细胞交互比例为11.6％，ABCG2+CD34+细胞交互比例为0。结论5-8F和6-10B中CD34+细胞比例与CSC理论中CSC的比例相符。CD34可以作为鼻咽癌CSC的候选标志物。