Objective To detect the expression and correlation of complement factor D (CFD) and adiponectin (ADPN) in the placenta tissues,serum and urine in pregnancy with preeclampsia. Methods It was selected 29 pregnant women with preeclampsia undergoing cesarean section (the preeclampsia group) and 30 normal full-term pregnant women with elective cae sarean section(the normal pregnancy group) admitted by the Department of Obstetrics of the First Affiliated Hospital of Chongqing Medical University from March to September in 2014. It was adopted Western blotting to detect the CFD and ADPN concentra-tions in placenta tissues and ELISA to detect the CFD and ADPN concentrations in serum and urine , calculating the two indicators′difference between the two groups and analyzing the correlation of the two. Results (1)The expression concentration of CFD protein of preeclamp sia patients was significantly increased in the placenta tissues compared to the normal pregnancy group(P<0.05 or 0.01). (2)The serum and urine concentrations of CFD in the normal pregnancy group were significantly lower than those in the preeclampsia group[(0.67±0.04)μg/mL,(43.27±16.59)ng/mL vs(1.83±0.59)μg/mL,(174.95±23.61)ng/mL, P<0.01];The serum and urine concentrations of ADPN in the normal pregnancy group were significantly higher than those in the preeclampsia group[(80.68±1.27),(9.29±1.33)ng/mL vs(13.16±2.01),(2.40±0.86)ng/mL,P<0.01]. (3) It showed a significantly negative correlation of CFD and ADPN concentrations in the placenta tissues ,serum and urine in pregnancy with preeclampsia (r=-0.82,-0.78,-0.63,P<0.05). Conclusion The abnormal changes of CFD and ADPN concentrations in the placental tissues , serum and urine in pregnant women may contribute to the pathology of preeclampsia ,and the two indicators may have synergistic effects. Taking advantage of combined detection of the CFD and ADPN concentrations in maternal serum or urine may be a promising method for prediction and diagnosis of preeclampsia.%目的：检测子痫前期孕妇胎盘组织、血清及尿液中补体因子D（CFD）以及脂联素（ADPN）的水平，分析二者的相关性及其临床意义。方法选取2014年3~9月在重庆医科大学附属第一医院产科住院行剖宫产术的子痫前期孕妇29例（子痫前期组）和正常足月妊娠行择期剖宫产术的孕妇30例（正常妊娠组），采用Western blotting检测正常妊娠组和子痫前期组孕妇胎盘组织中CFD、ADPN的表达水平。采用ELISA方法检测两组孕妇血清及尿液中CFD、ADPN水平，计算2个指标的组间水平差异，并分析二者的相关性。结果（1）胎盘组织中，与正常妊娠组比较，子痫前期组孕妇CFD表达水平显著升高，而ADPN水平显著下降，差异均有统计学意义（P<0.05或0.01）。（2）正常妊娠组孕妇血清及尿液中CFD水平为（0.67±0.04）μg/mL、（43.27±16.59）ng/mL，显著低于子痫前期组[（1.83±0.59）μg/mL、（174.95±23.61）ng/mL]，差异均有统计学意义（P<0.01）；正常妊娠组孕妇血清及尿液中ADPN水平[（80.68±1.27）、（9.29±1.33）ng/mL]显著高于子痫前期组[（13.16±2.01）、（2.40±0.86）ng/mL]，差异均有统计学意义（P<0.05）。（3）在子痫前期组孕妇的胎盘组织、血清及尿液中，CFD、ADPN水平均呈显著负相关（r=-0.82、-0.78、-0.63，P<0.05）。结论子痫前期孕妇胎盘、血清和尿液中CFD、ADPN水平的异常变化与子痫前期发病有关，二者可能存在协同作用。联合检测孕妇血清和尿液中CFD、ADPN水平有望成为预测及诊断子痫前期的一种新手段。
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