Objective To observe the difference in detection rate of Tuberculosis in different types of samples. Methods Sam pies were taken from 1 343 suspected patients with Tuberculosis to detect TB DNA by real time fluorescence quantitative PCR (FQ PCR) ,and meaaody 393 suspected patients to detect TB Ab in serum by Tuberculosis antibody colloidal gold diag nostic kit. Results The positive rate of bronchial fluid,bronchial brush biopsy,urine,sputum,pleural fluid and ascites in dif ferent types of samples from 1 343 suspected patients were 57. 14% ,21. 21 % ,13. 33% ,11. 23%,9. 09% and 3. 85% respec tively. The positive rate of sputum was lower than that of bronchial fluid and bronchial brush biopsy (X2 =14. 32 ,5. 87, P< 0. 05). 393 suspected tuberculosis patients,different types of samples were detected by FQ PCR and TB Ab colloidal gold di agnostic kit and the positive detection rates were 24. 43% and 47. 58% , respectively. There was significant difference be tween two methods (X2= 44. 73, P<0. 01) ,and the coincidence rate between two methods was 61. 57%. Conclusion The detection rate was highest in the bronchial fluid,and the detection rate was the lowest in ascites sample.%目的 讨论不同临床标本中结核分枝杆菌的检出率有何差异.方法 对1 343例疑似结核病患者的呼吸道和体液标本进行实时荧光定量PCR检测,同时对其中393例进行血清结核抗体检测.结果 1 343例疑似结核病患者不同临床标本经实时荧光定量PCR检测显示,支气管灌洗液检出率最高为57.14%,其次为支气管刷检物21.21%,尿液13.33%,痰液11.23%,胸腔积液9.09%,最低为腹腔积液3.85%.痰液与支气管灌洗液、支气管刷检物之间检出率差异具有统计学意义(χ2=14.32,5.87,P<0.05);393例不同临床标本同时进行实时荧光定量PCR和血清结核抗体检测,实时荧光定量PCR检出率为24.43%,血清结核抗体检出率为47.58%,两种方法的阳性率差异有统计学意义(χ2=44.73,P<0.01).血清结核抗体与实时荧光定量PCR检测的符合率为61.57%.结论 结核分枝杆菌在支气管灌洗液检出率最高,其次为支气管刷检物、尿液、痰液、胸腔积液,最低为腹腔积液.
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