探讨性别和年龄在慢性丙型肝炎患者外周血Toll样受体3和7基因表达中的影响

摘要

目的：观察不同性别和年龄慢性丙型肝炎（CHC）患者外周血单个核细胞（PBMC）中Toll样受体3（TLR3）和 Toll样受体7（TLR7）mRNA表达，探讨性别和年龄在CHC患者外周血TLR3和TLR7基因表达中的影响。方法选择 CHC患者115例，健康人群113例，取抗凝全血，实时荧光定量PCR检测TLR3和TLR7 mRNA表达。结果 CHC患者TLR3和TLR7表达量与健康人群相比，差异均有统计学意义（t＝38.73，6.16，P＜0.05）。CHC患者中非绝经期女性 TLR3和TLR7表达量与绝经期女性和青年男性相比，差异均有统计学意义（t＝61.210，6.464，P＜0.05；t＝24.166，26.266，P＜0.05）。CHC患者中老年男性 TLR3和 TLR7表达量与青年男性相比，差异均有统计学意义（t＝86.349，19.583，P＜0.05），而与绝经期女性相比，仅TLR3表达量差异有统计学意义（t＝122.941，P＜0.05）。不同性别和年龄 CHC患者的TLR3，TLR7基因表达量与 HCV-RNA载量均无相关性（｜r｜＜0.40，P＞0.05），但是青年女性 CHC患者 HCV含量均比青年男性和老年女性低，差异有统计学意义（t＝3.49，2.51，P＜0.05），老年男性患者 HCV含量比青年男性低，差异有统计学意义（t＝2.35，P＜0.05），老年男性和女性 CHC患者 HCV-RNA含量差异无统计学意义（t＝1.20，P＞0.05）。结论 性别和年龄与CHC 患者TLR 的表达有一定的关系，为临床合理有效治疗CHC 提供理论依据和新方法。%Objective Investigating the expression of TLR3 and TLR7 mRNA in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis C infection (CHC),to explore the effects of gender and age on Toll-like receptor expres-sion.Methods Peripheral blood was collected from 1 1 5 patients of chronic hepatitis C infection,1 1 3 healthy individuals.Ex-pression levels of TLR3 and TLR7 mRNA were detected by real-time quantitative PCR.Results The expression of TLR3 and TLR7 mRNA were significant difference between patients with CHC infection and healthy individuals (t=38.73,6.16, P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant difference between premenopausal fe-males with CHC infection,postmenopausal females and young males of CHC infection (t=61.210,6.464,P<0.05;t=24.166,26.266,P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant differences between old males and young males of chronic hepatitis CHC infection (t=86.349,19.583,P<0.05).The expression of TLR3 mRNA was significant differences between old males and postmenopausal females of CHC infection (t=122.941,P<0.05).There was no correlation between the expressions of TLR3 and TLR7 and HCV-RNA load of CHC patents of Gender and age dis-crepancies (|r|<0.40,P>0.05).The HCV-RNA load of premenopausal females was significant lower than young males and old females (t=3.49,2.51,P<0.05),the load of old males was lower than old females (t=2.35,P<0.05),however, there was no significant differences between old males and old females (t=1.20,P<0.05).Conclusion Gender and age dis-crepancies have a relationship with the expression of Toll-like receptors of patients with CHC infection,which may provide a theoretical basis and a new method for CHC.