耐碳青霉烯类鲍曼不动杆菌基因检测及同源性分析

摘要

Objective To detect and have homology analysis of resistant genes in clinical isolates of resistant Acinetobacter Bauman (CRAB).Methods Collected 40 clinical isolates of CRAB in Zhongshan Hospital of Traditional Chinese Medicine from July 2012 to December 2013,using PCR method for the amplification of the gene of carbon,sequence analysis.Applica-tion of ERIC-PCR to study the transmission mechanism.Results 40 strains of crab all isolates and detected containing oxa-51-like gene for 40 strains (100%),the gene oxa-23-like geneand for 38 strains (95%),were not detected in NDM,VIM, IPM,gene OXA-24-like and OXA-58-like.ERIC-PCR clustering analysis of similarity was greater than 0.8,that was the same colonies.The 40 strains of bacteria at a similar level were clustered into 33 taxa (clone).Conclusion The clinical iso-lates of CRAB mainly carry on the effective method of OXA-23-like,OXA-51-like,and ERIC-PCR for the homology analysis of Acinetobacter Bauman.%目的：临床分离出的耐碳青霉烯类鲍曼不动杆菌（CRAB）耐药基因检测及同源性分析。方法收集中山市中医院2012年7月～2013年12月临床分离40株CRAB，用 PCR法对碳青霉烯类基因进行扩增、序列分析；应用 ERIC-PCR研究其传播机制。结果40株 CRAB所有菌株且被检测到含有 OXA-51-like基因为40株（100％），OXA-23-like基因为38株（95％），未检出 NDM，VIM，IPM，OXA-24-like及 OXA-58-like基因；ERIC-PCR聚类分析相似大于0.8，可认为是同一克隆群；这40株菌在相似水平被聚为33个类群（克隆株）。结论临床分离的 CRAB主要携带碳青霉烯酶基因型为OXA-23-like，OXA-51-like和 ERIC-PCR是进行鲍曼不动杆菌同源性分析的有效方法。