A high efficient phenanthrene-degrading bacterial strain was isolated and screened from oil wastewater in a Lanzhou chemical plant and characterized it.The growth, different hydrocarbons degradation features, and phenanthrene biodegradation kinetics etc.of the strain were studied, combined with ultraviolet spectroscopy and GC-MS analysis.The major phenanthrene-degrading related genes of aromatic hydrocarbons metabolism were detected by the PCR amplification.The results showed that the strain F1 belonged to Acinetobacter johnsonii.The strain could normally grow on a basic medium containing 50-800 mg/L phenanthrene.Under the cultivation conditions as follows:30℃, p H 7.0, and salinity at 0.3％ (w/v) with 180 r/min of shaker, the degradation rate was at 43.57％5 d after the cultivation with final phenanthrene concentration at 100 mg/L, and the degradation process accorded with the first class kinetic feature.strain could also grow using biphenyl, naphthalene, anthracene and pyrene as sole carbon source.GC-MS analysis showed the strain had capability to degrade part of C10-C28 straight-chain alkanes.PCR amplification results indicated that the strain possessed genes in its genome such as catechol-1, 2-dioxygenase, benzoate dioxygenase, ferredoxin reductase, alcohol dehydrogenase, dihydroxy-acid dehydrogenase, aldolase, and redox protease genes.The results of the study provided basic data for the strain applying to restoration of the wastewater containing with phenanthrene and PAHs contaminated soil.%从兰州某化工厂石油废水中分离筛选出1株高效降解菲的细菌F-1并对其菌种进行鉴定, 结合紫外分光光度法及气相色谱-质谱联用 (GC-MS) 对菌株生长特性、不同烃类化合物降解特性及菲降解动力学等进行了研究, 利用PCR技术检测了芳香烃代谢相关基因.结果表明, 菌株F-1属于约翰逊不动杆菌 (Acinetobacter johnsonii), 可在终浓度为50～800 mg/L的含菲基础培养基中正常生长.在温度30℃、p H 7.0、盐度0.3％ (质量分数) 、转速180 r/min条件下培养5 d后菲 (终浓度为100 mg/L) 降解率为43.57％, 降解过程符合一级动力学特征.菌株F-1也能利用联苯、萘、蒽、芘为唯一碳源生长.GC-MS分析显示菌株对C10-C28部分直链烷烃具有较强的降解能力.PCR扩增结果表明, 菌株F-1基因组中存在邻苯二酚-1, 2-双加氧酶、苯甲酸盐双加氧酶、铁氧化还原蛋白还原酶、乙醇脱氢酶、二羟酸脱水酶、醛缩酶和氧化还原蛋白基因.研究结果为该菌株应用到含菲废水及多环芳烃污染土壤的处理和深度修复研究提供参考.
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