从鸟类苔藓和草叶巢材中分离出6株优势细菌和8株放线菌,采用传统表现型特征观察和16S rRNA基因序列分析法对其进行菌种鉴定.结果表明:草叶巢材中的优势细菌分别为霍氏肠杆菌(Enterobacter hormaechei)、麝香石竹假单胞菌(Pseudomonas caryophylli)和丁香假单胞菌(Pseudomonas syringae)；苔藓巢材中的优势细菌分别为苏云金芽胞杆菌(Bacillus thuringiensis)、阴沟肠杆菌(Enterobacter cloacae)和马氏棒杆菌(Corynebacterium matruchotii)；从2种巢材中分离出的8株放线菌均为链霉菌属(Streptomyces),其中4株分别为金色链霉菌(Streptomyces aureus)、红色淡紫灰链霉菌(Streptomyces rubrolavendulae)、胶样链霉菌(Streptomyces gelaticus)和灰色链霉菌(Streptomyces griseus),另外4株链霉菌有待于进一步鉴定.%Six dominant bacterial strains and eight actinomycetes strains were isolated from moss and grass/leaves bird nest materials ( BNM) and identified through general classification methods including colony characteristics, physiological and biochemical properties etc. And 16S rRNA gene sequence analyses. The results showed that the dominant bacterial strains from grass/leaves BNM were determined as Enlerobacter hormaechei, and Pseudomonas caryophylli, Pseudomonas syringae respectively; the dominant bacterial strains from moss BNM were Bacillus thuring-iensis, Enterobacter cloacae, and Corynebacterium malruchotii respectively. In addition, actinomycetes strains isolated from two BNM were Streptomyces. Of which four strains were Streptomyces aureus, Streptomyces rubrolavendulae, Slrep-tomyces gelations, and Streptomyces griseus. Another four remained to be identified further.
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