从湖北省大冶市古铜矿区域分离得到1株具有较高Fe2+氧化活性的嗜酸氧化亚铁硫杆菌SY,16SrDNA序列分析表明,该菌与Acidithiobacillus errooxidans(DQ 062116.1)的序列相似性最高.生理生化性质测定表明,菌株SY化能自养型,最适pH值为2.0,最适生长温度为30℃.菌株SY对Cu2+、Cd2+、Ni2+、Zn2+等重金属离子抗性的最低抑菌浓度分别为300、350、700和800 mmol/L,表明其对多种重金属离子均有很高的抗性.SY浸矿数据表明该菌对湖北大冶本土矿石浸出率为84.28％,高于其他地区矿石浸出率,在本土矿石生物浸出中显示出很大的优势.%A highly active ferrous iron oxidation Acidithiobacillus ferrooxidans strain SY,was isolated from an ancientcopper mining area in Daye,Hubei Province.Analysis of 16S rDNA sequence showed that the strain has high similarity to the sequence of A.ferrooxidans (DQ 062116.1).Physiological and biochemical determinations showed that the strain was a chemical energy autotrophically with the optimal growth pH at 2.0 and optimal growth temperature at 30 ℃.The MTC of the strain to resist Cu2+,Cd2+,Ni2 +,and Zn2+ were respectively at 300,350,700,and 800 (mmol/L),demonstrated it has high resistance against multiple heavy metal ions.Bioleaching data of SY showed its bioleaching rate on native ore was as high as 84.28％,higher than bioleaching rate on the ore from other mining areas,showing it has very high superiority on bioleaching native ore.The strain SY has great potential in the application of native minerals bioleaching.
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