婴幼儿淋巴结核病原体及多位点可变数目串联重复序列分析

摘要

The paper aims to determine the main pathogen causing tuberculous lymphadenitis in infants .A total of 79 lymph node biopsies from 73 infants age 0-3 were conducted Mycobacterium tuberculosis ( M . tuberculosis) identification , amplification of RD1 , RD9 and RD10 , and multiple-locus variable-number tandem repeat analysis (MLVA) .The results showed that the main pathogen of tuberculous lymphadenitis in infants was bacillus Calmette-Guérin (BCG) (95 .9% ) ,followed by M . tuberculosis (4 .1% ) .There were 4 genotypes including 3 independent genotypes (M . tuberculosis) and 1 genotype cluster analyzed by MLVA . The BCG strains isolated from clinical samples had no difference .%为了解婴幼儿淋巴结核的主要病原体及其分子生物学信息，对分离自73例0～3岁淋巴结核患儿的79份淋巴结穿刺液阳性培养物进行结核分枝杆菌鉴定、3个不同区域片段（RD1、RD9、RD10）扩增及多位点可变数目串联重复序列分析（MLVA）。结果显示，婴幼儿淋巴结核以卡介苗（BCG）感染为主（95．9％），其次为人型结核分枝杆菌感染（4．1％）。通过MLVA分离出4个基因型，3个为独立基因型、1个为成簇基因型。76株属成簇基因型，均为BCG ；3个独立基因型均为人型结核分枝杆菌。研究表明，BCG是引起婴幼儿淋巴结核的主要病原体，临床分离的BCG MLVA分型无差异。