刺老苞根皮黄酮类化合物对MC31E-E1成骨细胞OPG和RANKL mRNA基因表达影响的实验研究

摘要

目的:探讨刺老苞根皮黄酮类化合物对MC3T3-E1成骨细胞骨保护素(OPG)和核因子κB受体激活因子配体(RANKL)mRNA基因表达的影响.方法:体外培养MC313-E1成骨细胞,分别加入含0mol·L-1、10-8mol·L-1、10-7mol·L-1、10-6mol·L-1刺老苞根皮黄酮类化合物的培养液,48h及96h后分别提取细胞总mRNA,用RT-PCR方法分析OPG/RANKL的mRNA的表达,进行半定量分析.结果:培养48h后,与对照组比较,刺老苞根皮黄酮类化合物增强了OPGmRNA的表达,有显著性差异(P<0.05或P<0.01),VOPG/VRANKL比值增大;96h后,刺老苞根皮黄酮类化合物明显增强了OPG mRNA的表达,各浓度组与对照组相比,均有显著性差异(P<0.05或P<0.01),各浓度组VOPG/VRANKL比值增大,有显著性差异(P<0.05或P<0.01).结论:刺老苞根皮黄酮类化合物可能通过增加成骨细胞OPG的表达来抑制破骨细胞的分化和成熟,从而抑制骨吸收,达到治疗骨质疏松症的目的.%Objective: To explore the effect of Aralia echinocaulis Hand. Mazz flavonoids on expression of OPG and RANKL in MC3T3- E1 osteoblasts. Methods: The different Aralia echinocaulis Hand. Mazz flavonoids concentrations NA was prepared from osteoblasts after 48h and 96h, and mRNA expression of OPG and RANKL were detected by RT -PCR. Results:The mRNA expression of OPG in MC3T3-E1 osteoblasts significantly increase compared with that of 48h.Mter 96h,the mRNA expression of OPG significantly increased and the VOPG/VRANKL ratio was also decreased in the treated group compared with that of the control group. Conclusion: Aralia echinocauIis Hand. Mazz flavonoids sup-press the differentiatiou and maturate of osteoblast. Osteoporosis can be prevented and cured by Aralia echinocaulis Hand. Mazz flavonoids.