致敏树突状细胞治疗小鼠肝癌的实验研究

摘要

Objective:To investigate the role of dendritic cells (DC) pulsed with hepatoma cells in the treatment of mice liver cancer .Methods:Take BALB/c marrow cells from mice tibia and femur bone and use rmIL‐4 ,rmGM‐CSF , LPS ,RPMI‐1640 media in preparation of DC .Inactivated H22 hepatoma cells of mice mixed with DC(1∶3) cultured for preparation of DC(DC‐H22) sensitized by hepatoma cells .The experiment were divided into DC treatment group , DC‐H22 treatment group and saline‐treated control group .Two weeks later ,each group were observed changes in tumor volume ,tumor inhibition rate and survival after intraperitoneal injection ,respectively .Results:(1)Before treat‐ment tumor volume is substantially the same between the groups ,two weeks after different treatment ,tumor volume of DC treatment group and DC‐H22 treatment group compared with saline‐treated control group were significantly re‐duced ,indicating that DC and DC‐H22 treatment of mice liver cancer significantly inhibited subcutaneous tumor growth ,and H22 sensitized DC have more inhibitory effect on tumor growth .(2)After two weeks ,the inhibition rates were 31 .08% ,68 .30% in DC treatment group and the DC‐H22 treatment group ,the survival of the mice were also longer than the saline control group .Conclusion:Dendritic cells pulsed with mice hepatoma cells are better able to inhib‐it the grow th of liver cancer in mice ,they are important for treatment of liver cancer .%目的：探讨肝癌细胞致敏的树突状细胞（DC）治疗小鼠肝癌的作用。方法：取BALB／c小鼠胫骨和股骨的骨髓细胞，应用rmIL‐4、rmGM‐CSF、LPS、RPMI‐1640培养基制备DC。 H22小鼠肝癌细胞灭活后与DC按1∶3混合培养，制备肝癌细胞致敏的DC （DC‐H22）。实验分为DC治疗组、DC‐H22治疗组、生理盐水治疗组。各组分别腹腔注射后2周观察肿瘤体积的变化、抑瘤率及生存期。结果：（1）治疗前各组间的肿瘤体积基本相同，不同治疗2周后，DC治疗组、DC‐H22治疗组小鼠肿瘤体积较生理盐水治疗的对照组显著性减小，说明DC及DC‐H22治疗小鼠肝癌可以显著抑制皮下移植肿瘤的生长，而H22致敏的DC对肿瘤的抑制程度更大。（2）DC治疗组、DC‐H22组治疗2周后，其抑瘤率分别为31．08％、68．30％，小鼠的存活期亦较盐水对照组延长。结论：小鼠肝癌细胞致敏的树突状细胞能够更好地抑制小鼠肝癌的生长，对肝癌的治疗有重要意义。