高糖通过上调Axl受体表达促进心肌成纤维细胞增殖

摘要

Objective To investigate the effect of high glucose and normal glucose on the proliferation of cardiac fibroblasts and the role of Axl tyrosine kinase receptor in this process. Methods Cardiac fibroblasts of SD neonate rats (1 -3days) were seperated and the 2-4 passage cardiac fibroblasts were used for experiments. Cultured cardiac fibroblasts were incubated with high concentration glucose (25mmol/L) and normal concentration glucose (5mmol/L). After 48 h.the cell proliferation,cell cycle and Mrna expression of Axl were measured by MTT.flow cytometry and real - time PCR,respectively. Results ( 1) Compared with the group of normal glucose (5mmol/ L) .cell proliferation was significantly higher in the group of high glucose (25mmol/L) (OD490:0. 446 ±0.036 vs 0.358 ± 0. 023 ;P< 0. 01). ( 2) The Ct value of Axl in the group of high glucose was lower and its relative expression was higher than that in the group of normal glucose (Ct value:27.253 ±0.548 vs 28.996 ±0.579; relative expression: high glucose vs low glucose 1: 2. 63;P <0.05). (3)G2 + S + M% in the group of high glucose was higher than that in the group of normal glucose (36. 78 ±6.52 vs 26. 11 ± 5. 52 ;P < 0. 05 ). Conclusion High glucose could promote cardiac fibroblast cell proliferation by inducing up - regulation of Mrna of Axl. Axl receptor may play important role in diabetes cardiomyopathy.%目的 探讨不同浓度糖在心肌成纤维细胞增殖中的作用及Axl酪氨酸激酶受体在血糖调控心肌成纤维细胞增殖中的作用.方法 分别用不同浓度糖孵育乳鼠心肌成纤维细胞,48h后分别检测细胞增殖、细胞周期以及Axl mRNA的表达量.结果 (1)高糖组心肌成纤维细胞增殖显著高于正常糖组[OD490:正常糖组(5mmol/L)0.358±0.023；高糖组(25mmol/L)0.446±0.036；P＜0.01].(2)高糖显著促进Axl表达上调(Ct值:正常糖组28.996±0.579；高糖组27.253±0.548；相对表达量:1∶2.63;P ＜0.05).(3)高糖显著促进心肌成纤维细胞进入增殖周期(正常糖组G1％73.89±5.52；G2 +S+M％ 26.11±5.52;高糖组G1％63.22 +6.52；G2 +S+M％ 36.78 +6.52;P ＜0.05).结论 高糖能够通过上调Axl受体表达促进离体培养乳鼠心肌成纤维细胞增殖,Axl受体在糖尿病性心肌病变中有可能扮演重要作用.