巨细胞病毒感染患儿杀伤细胞免疫球蛋白样受体基因多态性分析

摘要

Objective To investigation whether the killer cell immunoglobulin like receptor ( KIR) gene polymorphism is associated with human cytomegalovirus(HCMV) infection. Methods Seventy six children infected with HCMV and 96 normal controls were enrolled to detect the KIR gene by PCR -SSP. Results The gene frequencies of KIR2DS1 ,KIR2DS2,KIR2DS5 were significantly higher in children infected with HCMV than in the healthy controls(X2 = 9. 145 , P = 0. 002;X2= 15. 167 , P = 0. 000 ;X2 = 4. 611 , P = 0. 032 ) . The rest of the gene frequencies was found to have no statistically significantly difference compared with healthy controls (P >0.05). Individuals who possessed three or more than three activating KIR genes were more patients than control subjects (63. 16% vs 32. 29% ,respectively,X2= 16. 273 ,P =0. 000). Conclusion KIR gene polymorphism is associated with HCMV infection and the increased activating KIR genes may be onset of the genetic factor.%目的 探讨杀伤细胞免疫球蛋白样受体(KIR)基因多态性与人巨细胞病毒(HCMV)感染是否存在关联.方法 采用PCR序列特异性引物(PCR-SSP)方法检测76例巨细胞病毒感染患儿和96例正常对照的KIR基因的多态性.结果 患儿组KIR2DS1、KIR2DS2、KIR2DS5基因频率显著高于健康对照组(x2=9.145,P=0.002；x2=15.167,P=0.000；x2 =4.611,P=0.032)；其余各KIR基因频率与对照组比较,差异均无统计学意义(P均＞0.05).具有3个或3个以上活化性KIR基因的个体在患儿组中占63.16％,在对照组中占32.29％,差异有统计学意义(x2=16.273,P=0.000).结论 KIR基因多态性与HCMV感染有关联,活化性KIR基因数目增多可能是HCMV感染后发病的遗传因素之一.