江西恶性血液病患者杀伤细胞免疫球蛋白样受体基因多态性研究

摘要

目的：了解江西恶性血液病患者杀伤细胞免疫球蛋白样受体(killer cell immunoglobulin-likereceptor，KIR)基因的多态性。方法采用PCR-SSP方法对江西地区62例恶性血液病患者进行基因分型，并与150例江西籍健康对照人群的KIR基因频率进行比较。结果共检出16个KIR基因和32种基因型，恶性血液病患者中2DL1、2DL2基因频率高于健康对照组，2DL3、3DL3、3DS1、3DP1基因频率低于健康对照组，差异均具有统计学意义(P<0.05)，其他KIR基因位点频率未发现统计学差异。结论 KIR基因的多态性与恶性血液病的发病机理可能有较为密切的关系。%Objective To investigate the polymorphism of killer cell immunoglobulin-like receptor (KIR)gene in patients with hematological malignancies in Jiangxi. Methods The sequence specific primer-polymerase chain reaction (SSP-PCR) method was used for genotyping of KIR in 62 patients with hematological malignancies and 150 healthy controls. Results A total of 16 genes and 32 different KIR genotypes in hematological malignancies were identified. Gene frequencies of 2DL1 and 2DL2 in patients were significantly higher than those in healthy controls, however, the frequencies of 2DL3,3DL3,3DS1 and 3DP1 were significantly lower than those in healthy controls (P<0.05). the frequencies of other KIR loci has no statistical difference between the two groups. Con-clusion There may be a close correlation between the polymorphism of KIR genes and pathogenesis of hematological malignancies.