靛玉红与DNA相互作用的荧光光谱研究

摘要

在模拟人体生理条件下,对抗癌药物靛玉红(IDB)与鲱鱼精DNA的相互作用进行了荧光特性研究.IDB的加入使DNA和盐酸小檗碱(BR)体系的荧光强度明显降低,且随着IDB加入量的增加,荧光猝灭现象越发严重,这表明嵌入DNA内部的BR分子受到了排挤,脱离DNA分子内的疏水环境进入了外部的亲水环境.同时,IDB与DNA作用后的吸收光谱发生了红移和减色效应,说明二者之间存在嵌插性的结合.磷酸盐效应及盐效应实验排除了静电和沟槽这两种结合方式.Scatchard方程进一步验证了IDB与DNA之间只存在单一的嵌插作用方式,并由此得出两者的结合常数.%Under simulated physiologic conditions,the interaction between anticancer drug indirubin(IDB) and herring sperm DNA was studied by fluorescence spectroscopy.The addition of IDB reduced the fluorescence intensity of DNA and berberine hydrochloride(BR) system.And the fluorescence quenching was enhanced with the increase of IDB addition amount.The result indicated that the BR molecules intercalated into DNA were squeezed out from the hydrophobic environment within DNA into the outer hydrophile site.After binding to DNA,the UV-Vis spectra of IDB showed red shift and peak decrease,indicating the insert binding between them.Furthermore,both the electrostatic and groove binding were eliminated by phosphate effect and salt effect experiments.The conclusion was confirmed that only intercalation existed between IDB and DNA,and their binding constant was obtained by Scatchard equation.