钙黏蛋白相关基因在慢性粒细胞白血病中的表达及其作用

摘要

目的 研究钙黏蛋白(Cad)相关基因在慢性粒细胞白血病(CML)不同发展时期患者骨髓单个核细胞(BM-MNC)中的表达,探讨Cad相关基因在CML发展中的作用.方法 收集2013年12月至2014年12月长治医学院附属和济医院收治的48例CML患者的骨髓标本,其中慢性期29例,进展期19例.应用实时定量反转录-聚合酶链反应(qRT-PCR)法检测所有患者BM-MNC中上皮型钙黏蛋白(E-Cad)和神经元型钙黏蛋白(N-Cad)基因的相对表达量,并对结果进行分析.结果 CML患者BM-MNC中均可检测到E-Cad、N-Cad基因的表达.E-Cad基因在CML进展期患者中的相对表达量低于慢性期患者(0.20±0.35比1.19±0.87,P＜ 0.01),而N-Cad基因在进展期患者中的相对表达量高于慢性期患者(0.89±0.45比0.57±0.47,P＜0.05).E-Cad基因表达与外周血原始细胞比例呈负相关(r=-0.705,P＜ 0.01).结论 E-Cad、N-Cad基因表达与CML的疾病进展相关,其表达水平可作为评估疾病进展风险的指标之一.%Objective To explore the expression of cadherin (Cad) genes in patients with chronic myeloid leukemia (CML),and to elucidate the significance of Cad genes in the development of CML.Methods E-Cad and N-Cad gene expression levels in bone marrow mononuclear cells (BM-MNC) from 48 CML patients (29 in chronic phase,19 in progressive phase) were detected by real time quantitative polymerase chain reaction (qRT-PCR).Results Gene expression of E-Cad and N-Cad was detectable in CML BM-MNC.E-Cad gene expression level was lower in progressive CML than that in chronic CML (0.20 ± 0.35 vs 1.19 ± 0.87,P ＜ 0.01),while N-Cad was highly expressed in progressive CML than in chronic CML (0.89 ± 0.45 vs 0.57 ± 0.47,P ＜ 0.05).E-Cad gene expression level was negatively corrclatcd with thc pcrccntagc of peripheral blood progenitor cells (r =-0.705,P ＜ 0.01).Conclusion E-Cad and N-Cad gene expression correlates with the progression of CML,and might be used as an evaluation index for disease development.