miR137及其靶基因Kruppel样转录因子12在多发性骨髓瘤中的表达及预后意义

摘要

目的：探讨miR137的靶基因Kruppel样转录因子12（KLF12）对多发性骨髓瘤（MM）预后的影响。方法通过软件预测miR137的靶基因，应用基因表达谱（GEP）分析KLF12基因是否为MM预后因素，构建miR137过表达的MM细胞株NCI-H929，应用实时定量聚合酶链反应、Western blot分别检测KLF12基因和蛋白在细胞株中的表达。结果软件预测 miR137的靶基因有 MITF、BUE2H、SH3BP5、KLF12。对GEP芯片中患者数据进行分析，高表达KLF12的患者455例，其中75例（16.5%）死亡，低表达KLF12的患者104例，其中25例（24.0%）死亡。miR137过表达的细胞株NCI-H929中KLF12较高。MM患者的miR137水平和KLF12的表达呈正相关（r＝0.67，P＝0.01）。结论 miR137-KLF12是判断MM预后的重要指标。%Objective To explore the prognostic impact of miR137 target gene Kruppel-like transcription factor 12 (KLF12) in multiple myeloma (MM). Methods The target genes of miR137 were predicted by software. The GFP analysis of KLF12 and the prognosis of MM were constructed. Overexpressing miR137 in MM NCI-H929 cell line was also constructed. Real-time qPCR and Western blot were used to detect the expression of KLF12 in this cell line. Results The target genes of miR137 were MITF, BUE2H, SH3BP5 and KLF12. High expression of KLF12 in 455 patients included 75 patients (16.5 %) died, 104 patients with low expression of KLF12, and 25 patients (24.0 %) died, but no significance was detected in the different subgroups. KLF12 expression was higher in MM NCI-H929 cell line with miR137 over expression. The expression of miR137 was positively correlated with the expression of KLF12. Conclusion miR137-KLF12 is an important index to judge the prognosis of MM.