生存素及基质金属蛋白酶-9对胎盘发育的调节

摘要

Objective To investigate the role of survivin and matrix metalloproteinase-9 (MMP-9) in regulating the placental development. Methods Real-time flurescent quanti-tive polymerase chain reaction was used to detect the expression of survivin and MMP-9 mRNA in chorion and placenta, including 20 normal chorionic villi in early pregnancy, 15 normal placental tissues in second trimester pregnancy, 20 normal placental tissues in third trimester pregnancy. Results In normal pregnancy, the expression of survivin mRNA and MMP-9 mRNA were statistically decreased with the progress of pregnancy (P <0.05). Conclusion Survivin may regulate placental trophoblast cell apoptosis. The expression of survivin is consistent with the normal placental development, and survivin is an important factor in placental development. MMP-9 is involved in trophoblast cell invasion. It is also important in the regulation of placental development.%目的 研究生存素及基质金属蛋白酶-9(MMP-9)对胎盘发育的调节作用.方法 采用荧光实时定量多聚酶链式反应方法检测正常早期妊娠绒毛组织(20例)、中期妊娠胎盘组织(15例)及晚期妊娠胎盘组织(20例)中生存素及MMP-9 mRNA的表达.结果 正常妊娠早、中、晚期胎盘组织生存素mRNA及MMP-9 mRNA表达随孕周增加而降低,差异有统计学意义(P＜0.05).结论 生存素可调节正常胎盘滋养细胞凋亡,其表达变化与胎盘发育过程相吻合,是胎盘发育形成过程中重要的调控因子；MMP-9作为胎盘滋养细胞侵袭的重要调控因子,是胎盘正常发育的重要因素.