Objective To investigate the STAT4 mRNA expression levels,the serum levels of IL-12 and IFN-γ in peripheral blood of patients and normal individuals,and to explore its possible role in the pathogenesis of SLE.Methods STAT4 expression of SLE patients and normal controls were analyzed with QPCR technique.ELISA was applied to determine the serum levels of IFN-γ and IL-12.The data were analyzed with the Plink 1.06 software and spss 16.0.Results 1.The expression levels of STAT4 mRNA of SLE patients were significantly higher than those of control groups (P ＜ 0.01) There was a correlation between STAT4 and SLEDAI (r =0.385,P ＜ 0.05).2.The G-allele of rs10181656 increased the expression of STAT4 mRNA.3.The serum levels of IFN-γ and IL-12 of SLE patients were significantly higher than those of normal controls (P ＜ 0.01).The serum levels of IFN-γ and IL-12 were positively correlated to SLEDAI (P ＜ 0.05).4.The expression levels of STAT4mRNA showed a positive correlation with IFN-r (r =0.335,P =0.009) and IL-12 (r =0.401,P =0.002),and showed a positive correlation with serositis,hematological,kidney damage and butterfly rash (P ＜ 0.05) Conclusions 1.The expression levels of STAT4 mRNA of SLE increase and correlate with SLEDAI,so the expression levels of STAT4 might be used as a referential indicator,suggesting STAT4 may play a role in development of SLE and correlate with the severity of SLE.2.The serum levels of IFN-r and IL-12 increase,and show a positive correlation with SLEDAI,suggesting that IFN-r,IL-12 may be as parameters of SLE activity.According to the mRNA expression levels of STAT4 associated with IFN-r and IL-12,we conclude that STAT4 may have active effect on IFN-r and IL-12 in the gene level.STAT4 may regulate IFN-r,IL-12 and enhance the mRNA expression of the two cytokines.%目的 研究SLE患者STAT4的表达与患者血清中细胞因子IL-12、IFN-γ的关系,探讨它们在SLE发病中的作用与狼疮疾病活动程度的关系.方法 应用QPCR检测患者血液中STAT4的表达.应用ELISA方法检测正常对照组与SLE患者血清中IL-12、IFN-γ的水平.所有数据资料用Plink l.06及SPSS16.0软件进行统计分析.结果 (1) SLE病人STAT4 mRNA的相对表达量高于正常对照组(P＜0.01),STAT4表达水平与SLEDAI评分成正相关(r=0.385,P＜0.05)； (2) rs10181656 G等位基因的突变与STAT4 mRNA的表达水平升高有关；(3) SLE组IFN-r、IL-12含量高于正常组(P＜0.01)；IFN-r、IL-12含量与SLEDAI评分呈正相关(P＜0.05)；(4) STAT4 mRNA的表达与IFN-γ(r=0.335,P＜0.05)、IL-12 (r=0.401,P＜ 0.05)显著正相关,与其他临床及实验室指标蝶形红斑(r=0.471,P＜0.000)、粘膜溃疡(r=0.552,P=0.000)、浆膜炎(r=0.311,P=0.014)、肾损害(r=0.247,P=0.042)、血液损害(r=0.256,P=0.003 7)成正相关(P＜0.05).结论 (1)SLE患者中STAT4基因的表达水平增高并与SLE疾病活动相关,可以作为监测病情活动性的检测指标； (2)SLE患者的IFN-γ、IL-12异常高表达,而且与SLEDAI评分呈正相关,IFN-γ、IL-12可以作为判断SLE疾病活动的参考指标.STAT4对IL-12、IFN-γ的表达有正相调节作用,在基因水平上调控IL-12、IFN-γ并促进其mRNA的表达.
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