Objective To investigate the role of chemokine SDF-1 and its receptor CXCR4 in malignant glioma cell proliferation, invasion and migration, so as to provide basis for clinical prevention and treatment. Methods Immunohistochemistry was used to detect the CXCR4 expression in malignant glioma cells. MTT assay was used to test different concentration of SDF-1 promoted in vitro proliferation of C6 cells malignant glioma. CXCR4 receptor inhibitor AMD3100 and SDF-1 were co-cultured to test cell proliferation and CXCR4 resistance in malignant glioma by observing the above indexes. The cell migration and invasion ability of C6 in different groups was tested by transwell migration and invasion assay. Results CXCR4 expressed positively in malignant glioma cell line C6. SDF-1 promoted C6 cells proliferation, invasion and migration in vitro. Anti-CXCR4 McAb could inhibit the proliferation, invasion and migration in a dose-dependent manner. AMD3100 inhibited the effects induced by SDF-1. Conclusion The role of SDF-1/CXCR4 in malignant glioma cell lines proliferation, invasion and migration in vitro is related to invasion and migration of malignant glioma, and may be a new therapeutic target for glioma.%目的 探讨趋化因子SDF-1及受体CXCR4在恶性胶质瘤细胞中的增殖、迁移及侵袭的作用,为临床治疗提供依据.方法 免疫组化法检测CXCR4在恶性胶质瘤C6细胞中的表达；用MTT法分别检测不同浓度的趋化因子SDF-1促恶性胶质瘤C6细胞体外增殖,并应用CXCR4受体抑制剂AMD3100与SDF-1共培养细胞观察增殖情况及抗CXCR4单克隆抗体在恶性胶质瘤中上述指标的变化.通过细胞迁移实验和细胞侵袭实验评价不同处理组C6细胞的迁移和侵袭能力.结果 CXCR4在恶性胶质瘤C6细胞系表达呈阳性.SDF-1可以促进恶性胶质瘤C6细胞的体外增殖、迁移和侵袭,而抗CXCR4单克隆抗体可以有效抑制其增殖、迁移和侵袭,并呈浓度依赖性.AMD3100可以抑制SDF-1的诱导作用.结论 SDF-1/CXCR4轴在恶性胶质瘤细胞株的体外增殖、迁移及侵袭中的作用,可能与恶性胶质瘤的侵袭和转移有关,为治疗恶性胶质瘤提供新的治疗策略.
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