应用流式细胞术快速测定细胞周期的DNA一步法

摘要

目的 探索能通过流式细胞术快速测定细胞周期的方法.方法 培养的小鼠成纤维细胞分别采用DNA一步法和溴化丙啶(PI)标准染色流程染色,应用流式细胞仪检测细胞G1期、S期和G2/M期的DNA含量,比较2种方法的测定结果.结果 2种染色方法标记的小鼠成纤维细胞呈现完整的细胞周期峰,G1含量分别为65.85％和66.54％,S期含量分别为13.45％和13.32％,G2/M期含量分别为20.71％和20.15％,结果差异无统计学意义(P＞0.05).结论 DNA一步法能通过流式细胞术快速准确地测定细胞周期.%Objective To explore the method that can rapidly analyze cell cycle by flow cytometer.Methods Mouse fibroblast cells stained with DNA one-step method and Bromide (PI) standard dyeing process were measured by flow cytometer for the percentages of cells in G1,S and G2/M phases.The results were analyzed and compared.Result The complete cell cycle of mouse fibroblast cells stained with DNA one-step method or Bromide bromide (PI) standard dyeing process was showed entirely,the percentages of cells in G1 were 65.85％ and 66.54％,in S were 13.45％ and 13.32％,and in G2/M,20.71％ and 20.15％,respectively.The results from the two different methods showed no significant difference (P＞0.05).Conclusions The DNA one-step method can quickly and accurately analyze cell cycle by flow cytometer.