Aim:We propose to increase citrate regulation through the combination the multi-target effect of Ds and single target effect of LiC,enhance the prophylaxis of CaOx stones and clear its possible mechanism.Methods:90 adult male Wistar rats were randomly divided into nine groups (Normal,Pla-cebo,LDs,MDs,HDs,LiC,LDs+LiC,MDs+LiC,HDs+LiC).After one week of acclimatization, the rats were fed by containing 5% ammonium oxalate (AmOx)feed for one week,and then given the different does aqueous extracts(1ml/time,Bid).On the 21st day of experimental period,the rats were placed in separate metabolic cages to collect 24 -h urine,and then both blood and kidneys were ob-tained.Samples were used to various determinations involving physical and biochemtry examination, crystalluria,CaOx deposition evaluation and pathological examination.Results:Serum Li was only de-tected in Lic and combination therapy groups,and showed a gradually increasing trend.Moreover,serum Li level was 1.38 ±0.41 mmol/L in high dose combination therapy group.Urinary pH value,citrate, and calcium concentration significantly increased.Of these,urinary citrate level increased 53% and 24.3% compared with Ds and LiC groups,respectively.There was 1-fold increase in urinary volume and 1-fold decrease in Ox excretion.Conclusion:The combination therapy middle dose Ds and LiC indicates obvious synergistic action on urinary citrate,which can increase 4.15 folds compared with normal.%目的：将金钱草抑制结石形成多作用靶点和碳酸锂单作用靶点相结合，探讨预防大鼠肾结石形成及作用机制．方法：90只成年雄性Wistar大鼠随机分入九组（正常、对照、低中高剂量金钱草组、碳酸锂组和低中高剂量金钱草联合碳酸锂组）．经一周适应环境，5％草酸铵饲料喂养实验组大鼠一周后，以不同浓度水煎剂灌胃（1 mL/次，2次/天）．第21天留取尿液/24 h、血及肾标本．检测血、尿标本各项理化指标，观察尿结晶、肾结晶沉积和病理改变．结果：血清锂仅在碳酸锂及联合治疗组检测到，且呈逐渐增高趋势，高剂量金钱草联合治疗组为1.38±0.41 mmol/L．中高剂量联合治疗组的尿柠檬酸水平较中高剂量金钱草组增高53％，较碳酸锂组增高24.3％，尿量/24 h增加1倍，尿草酸水平降低1倍．结论：中剂量金钱草联合碳酸锂组对尿柠檬酸浓度表现出显著的协同调节作用，使尿柠檬酸水平较正常大鼠升高4.15倍．
展开▼
