五味子乙素对HepG2细胞凋亡及Hsp-70基因表达的影响

摘要

Objective To explore the inhibition effect of different concentration schisandrin B ( Sch B ) on HepG2 and its mechanism.Methods Cultivating HepG2 cells with 10,25,50,75,100μmol/L Sch B respectively,the MTT method was employed to screened the low toxicity concentration of Sch B .After cultivating with the above gradient con-centration schisandrin B ,the stereochimical structure of HepG 2 cells were observed by invert microscope and its activi-ty was detected by MTT method and western blot was utilized to observe the expression of Hsp -70.The apoptosis con-dition and cell cycle were detected by flow cytometory .Results Sch B had anti-proliferation effect on HepG2 cells growth in dose-dependent manner .The results of flow cytometry showed that the percentage of HepG 2 in sub-G1 phase in 10 μmol/L and 25 μmol/L Sch B group were significantly increased than that in negative control groups ( P<0.05).Conclusion The mechanism of HepG2 cell apoptosis induced by Sch B is probably associated with the down-regulation of Hsp-70 protein expression .The best concentration was 10μmol/L when the Sch B ,as the immuno-modulator ,is combined with other anticancer drug .%目的：探讨五味子乙素（ Sch B ）对人肝癌HepG2细胞的抑制作用，并研究其可能机制。方法10、25、50、75、100μmol／L Sch B作用于HepG2细胞，通过MTT法筛选低毒性的Sch B浓度，梯度浓度Sch B作用HepG2细胞48 h后，倒置显微镜下观察细胞形态；MTT法检测细胞活性；Western blot法检测Sch B作用HepG2细胞48 h后不同浓度组热休克蛋白-70（ Hsp-70）的表达情况；流式细胞仪检测细胞凋亡及周期改变。结果Sch B能够抑制HepG2细胞的生长，并呈浓度依赖性；流式细胞术结果显示10、25μmol／L Sch B组细胞在Sub-G1期明显高于阴性对照组（P＜0．05）。结论 Sch B促凋亡机制可能与下调Hsp-70蛋白的表达有关；如做免疫调节剂与其他抗癌药物联合使用最佳给药浓度为10μmol／L。