玉米自交系Ⅱ型胚性愈伤组织诱导及遗传转化初报

摘要

Callus Ⅱ were initiated from the bud of inbred line 340 and immature embryos of inbred line 4112 of maize. Callus Ⅱ were bombarded with Bar gene and GUS activity was detected.%以玉米自交系340芽尖、4112未成熟幼胚为试验材料，诱导出良好的Ⅱ型胚性愈伤组织；用这2种材料的Ⅱ型胚性愈伤组织为受体，通过基因枪轰击法将Bar基因转入玉米细胞，然后用GUS基因检测，结果为阳性。