龙牙楤木SE基因克隆与植物表达载体构建

摘要

The squalene epoxidase (SE) gene from Aralia elata (AeSE) was amplified by RT-PCR.The result showed that the full-length cDNA of AeSE gene (GenBank accession Number: GU354314) is 1 682 bp and contains a 1 644 bp open reading frame (ORF) encoding a polypeptide of 547 amino acids.The SE proteins from Aralia elata, Panax ginseng, Panax notoginseng and Gynostemma pentaphyllum showed more than 90％ identities based on amino acid sequences alignment. The plant expression vector pCAMBIA 3301 - AeSE was constructed.%采用RT-PCR的方法,从龙牙木忽木中克隆到鲨烯环氧酶(SE)基因,该基因的cDNA全长为1 682 bp,含有1 644 bp的开放阅读框(ORF),编码547个氨基酸,将所得的序列提交GenBank数据库,登录号为GU354314.序列分析结果表明:龙牙楤木SE基因的氨基酸序列与人参、三七、绞股蓝的同源性>90%,并构建了该基因的植物表达载体pAeSE.