细胞骨架抑制剂对狂犬病病毒感染人神经瘤细胞的影响

摘要

To study the inhibitory effects of cytoskeleton inhibitors on rabies virus (RABV)infection in vitro,colchicine (Col)and cytochalasin D (CytoD)were chosen,their suitable concentration on neurob-lastoma (SH-SY5Y)was screened,and their effects on RABV earlier infection and replication were eval-uated .The results showed that aggravated damage of microtubules and actin filaments was closely associ-ated with the highly elevated concentration of inhibitors .When Col was at 0.065 mmol/L and 0.1 3 mmol/L,respectively,and CytoD at 0.25μg/mL and 0.50μg/mL,no significant structural changes of microtubules and actin were observed compared with the control (P>0.05);the two cytoskeleton in-hibitors demonstrated inhibitory ability in RABV infection and replication,RABV titer and Q-PCR of G gene mRNA confirmed that CytoD played a more important role in early RABV infection than Col,howev-er,a more significant role of the Col was proved in RABV replication than CytoD .All these results pro-vided experimental data for cytoskeleton associated RABV pathogenesis .%为检测细胞骨架抑制剂对狂犬病病毒（RABV）感染细胞的抑制作用，筛选了秋水仙素（Col）和细胞松弛素D（CytoD）2种骨架特异性抑制剂在人神经瘤细胞SH-SY5 Y上的适宜浓度，探讨其对RABV感染及复制的影响。结果表明：随着细胞骨架抑制剂浓度的升高，微管和微丝的破坏加剧；当Col浓度分别为0.065 mmol/L和0.130 mmol/L及CytoD浓度分别为0.25μg/mL和0.50μg/mL时，微管、微丝结构及细胞活力均与对照组无显著差异（P＞0.05），2种骨架抑制剂对RABV的感染及复制均有抑制作用。通过对RABV培养物的OD值和G基因mRNA的定量检测，发现CytoD对RABV感染的抑制作用较Col更明显，Col对RABV复制的抑制作用较CytoD更明显。