电针结合运动疗法对急性脊髓损伤大鼠Cdc42因子表达的研究

摘要

Objective:To investigate the electric -acupunture ( EA) combined with BWSTT by evaluating the expression of Cdc42. Method:90 adult maLe SD rats were randomized into the sham group ( A group) , the model group ( B group) , Jia-Ji EA group ( C group) ,EA with BWSTT group ( D group) and Fausidil group( E group) .Spinal cords injury was made by NYU impactor on T9-T10 spinal segment.Immunohistochemical techniques detect the expression of Cdc42 in lesion segment at each time points.Results:Cdc42 expression stayed a low-level in the sham group.Expression after SCI was gradually increased, and reached a peak at 28d.The two treatment groups and drug group compared with the model group had a significant differences at 7d, 14d, 28d (P<0.05).The differ-ence between the two treatment groups was significant at 28d (P<0.05).Conclusions:EA and BWSTT therapy may be increase the expression of Cdc42 to promote the recovery of motor function in rats by Rho /ROCK signaLing pathway.For the long-term rehabili-tation after SCI, the EA combined with BWSTT superior than EA group on the recovery of motor function after SCI.%目的：本实验通过检测脊髓损伤后Cdc42的蛋白表达来探讨电针结合减重步行训练疗（ BWSTT）法的治疗机制。方法：选用健康成年雄性SD大鼠90只，随机分为假手术对照组（ A组）、模型对照组（ B组）、夹脊电针观察组（ C组）、夹脊电针结合减重步行训练观察组（ D组）、药物组（ E组）。急性脊髓损伤（ SCI）模型采用美国NYU脊椎冲击损伤仪致大鼠T9－T10段脊髓急性中度损伤。采用免疫印迹和免疫组化技术检测各时间点损伤段脊髓Cdc42的表达。结果：在两项检测技术中，假手术对照组的蛋白表达一直处于稳定低水平状态。 SCI术后表达呈逐渐增加的趋势，28 d时达到高峰，两组观察组及药物组与模型组相比在7d、14d、28d均有显著性差异（P＜0．05），两组观察组之间在28d时具有显著性差异（P＜0．05）。结论：电针结合BWSTT疗法可能通过增加Rho／ROCK激酶信号通路中下游因子Cdc42的表达，促进大鼠运动功能的恢复；从损伤后长久的复健来看，电针结合BWSTT疗法对SCI后运动功能的恢复上优于电针观察组。