武汉地区肝癌患者中HBV基因型分析

摘要

Objective： HBV infection state of primary hepatocellular carcinoma and HBV genotypes were investigated.Methods： The HCC patient serum was determined by special primer with PCR.Results： The positive rate of HBV was 88% in HCC（44/50） and HBV C genotype was 68%（30/44）,HBV B genotype was 32%（14/44）.Conclusion： Primary hepatocellular carcinoma in Wuhan area is mostly the HBV infection patients,and most of them are HBV C-type infection patients.%目的：分析武汉地区原发性肝癌患者HBV感染情况及基因型分析。方法：利用HBV检测引物及分型引物对肝癌患者血清进行PCR,通过电泳检测PCR特异性产物。结果：50例患者血清中44例HBV DNA呈阳性,占总数的88%;其中30例为C型占68%,14例为B型占32%。结论：武汉地区原发性肝癌患者多为HBV感染患者,而且以HBV C型感染为主。