高效液相色谱法同时测定杜仲叶中4种有效成分的含量

摘要

目的：测定杜仲叶中4种有效成分的含量。方法采用HPLC测定绿原酸、槲皮素、山奈酚、芦丁的含量，色谱柱为WondaSil C18 Superb色谱柱（4.6 mm ×250 mm，5μm），流动相：0.4%磷酸溶液（A）-甲醇（B）进行梯度洗脱，流速1 ml/min，进样量10μl，检测波长262 nm。结果绿原酸的质量在0.825~4.123μg（R2=0.9999）范围内呈良好线性关系，平均回收率为101.89%，RSD为0.49%；槲皮素的质量在0.165~0.824μg（R2=0.9998）范围内呈良好线性关系，平均回收率为99.88%，RSD为2.64%；山奈酚的质量在0.021~0.104μg（R2=0.9999）范围内呈良好线性关系，平均回收率为100.0%，RSD为0.89%；芦丁的质量在0.002~0.012μg（R2=0.9991）范围内呈良好线性关系，平均回收率为100.0%，RSD为0%。结论本方法简便、快速、准确、重复性好，适用于杜仲叶中各成分的含量测定。%Objective To determine the four effective components in Eucommia folium. Methods HPLC was employed to determine the contents of four effective components:chlorogenic acid,quercetin,kaempferol,rutin. The chromatogram colume was WondaSil C18 Superb(4.6 mm × 250 mm,5μm),gradiently eluting with methanol as mobile phase A and 0.4%phosphoric acid as phase B at a flow rate of 1 ml/min. The injection volumn was 10μl. Detection wavelength was set at 262 nm. Results Chlorogenic acid was linear in the range of 0.825-4.123μg with correlation coefficient of 0.9999. The average recovery was 101.89%with RSD 0.49%(n=6). Quercetin was linear in the range of 0.165-0.824μg with correlation coefficient of 0.9998. The average recovery was 99.88%with RSD 2.64%. Kaempferol was linear in the range of 0.021-0.104μg with correlation coefficient of 0.9999. The average recovery was 100.0% with RSD 0.89%. Rutin was linear in the range of 0.002-0.012 μg with correlation coefficient of 0.9991. The average recovery was 100.0%with RSD 0%. Conclusion The method is convenient,fast,accurate and practicable. It is suitable for content determination of components in Eucommia folium.