小麦黄素水剂中小麦黄素含量的反相高效液相色谱法测定

摘要

建立反相高效液相色谱测定小麦黄素水剂中小麦黄素 (麦黄酮 )含量的方法。采用 Spherisorb C18柱，以甲醇－水－乙酸（体积比 65∶ 35∶ 2）为流动相，流速 1 mL/min，检测波长 352 nm。小麦黄素在 5～ 100 mg/L范围内质量浓度与峰面积呈良好的线性关系，相关系数 r=0.998 6，该法灵敏、准确，重复性好，平均回收率为 97％。该法为测定小麦黄素水剂中的小麦黄素含量提供了可靠的手段。%A method for the determination of content of tricin in water -base tricin was established by reversed -phase high performance liquid chromatography. Chromatographic separation was performed with spherisorb C18 column with an eluent consisting of methanol-water-acetic acid(65∶ 35∶ 2 by volume), and the eluate was monitored spectrophotometrically at λ max=352 nm. The calibration curve was linear over the range of tricin mass concentration from 5 mg/L to 100 mg/L. The recovery rate is 97％ (n=5). The method is rapid, sensitive, accurate and repeatable. It was successfully applied to assay the tricin amount in water -base tricin.