气相色谱-正化学源质谱法测定家用橡胶手套中7种N-亚硝胺及其前体物的迁移量

摘要

A gas chromatography - positive chemical ionization mass spectrometric ( CC - PCI/MS)method was developed for the determination of migration levels of 7 N-nitrosamines and N-nitrosatable substances including N-nitrosodimethylamine ( NDMA), N-nitrosodiethylamine ( NDEA ), N-nitrosodi-n-propylamine ( NDPA), N-nitrosodi-n-butylamine ( NDBA ), N-nitrosopiperidine ( NPIP), N-nitrosopyrrolidine(NPYR) and N-nitrosomorpholine (NMOR) from household rubber gloves by using Sep-Pak AC2 connecting to Sep-Pak Dry cartridges. The developed method was simple, rapid, sensitive and accurate. Its quantitation limits for N-nitrosamines and N-nitrosatable substances were 1.25 μg/kg and 5.0 μg/kg, respectively. The total release values for the 7 N-nitrosatable substances were 6. 96- 168. 46 μg/kg, in which NDEA, NDMA and NDBA were found most frequently in 13 analysed household rubber gloves. The risk exposure levels of seven N-nitrosamines from household rubber gloves were in the class of 1.81 -43.80 μg per person in a year, which would bring a potent tial risk to people, especailly to some occupational population.%该文用Sep-Pak AC2串联Sep-Pak Dry cartridges固相萃取小柱对家用橡胶手套迁移提取液中的N-亚硝基二甲胺(NDMA)、N-亚硝基二乙胺(NDEA)、N-亚硝基二丙胺(NDPA)、N-亚硝基二丁胺(NDBA)、N-亚硝吗啉(NMOR)、N-亚硝基吡咯烷(NPYR)和N-亚硝基哌啶(NPIP)7种对人体具有致癌活性的N-亚硝胺及其前体物进行分离净化,采用气相色谱-正化学源质谱法(GC-PCI/MS)对其进行定性定量分析.N-亚硝胺及其前体物的定量下限分别为1.25、5.0μg/kg.方法灵敏度高,流程简单,适于批量样品的快速分析.13种家用橡胶手套样品中,7种N-亚硝胺的总迁移量为6.96～168.46ug/kg,其中NDEA、NDMA和NDBA的检出频率最高,占N-亚硝胺总迁移量的比重最大.