A quantitative method was established for the determination of A -tetrahydrocannabinol in cannabis using high performance liquid chromatography. Methanol was selected as the solvent to extract tetrahydrocannabinol from cannabis plant or resin. The separation of tetrahydrocannabinol was performed on a Shimadzu ODS - SP column( 150 mm ×4. 6 mm, 5μm) using water - acetonitrile as mobile phase by gradient elution. The primary quantitative wavelength of UV detector was set at 210 nm and the auxiliary one was at 195 nm or 220 nm. Flow rate was set at 1 ml/min, column temperature was 40 ℃ and injection volume was 10 μL. Under the optimal conditions, good linearity was achieved over the range of 0. 5 - 100. 0 mg/L, with correlation coefficients (r2) more than 0. 999 99. The limit of detection(S/N ≥.3.3) was 0. 12 mg/L and the limit of quantitation(S/N≥10) was 0.40 mg/L. Using this method to detect a cannabis resin sample, the result of uncertainty evaluation on Δ9-tetrahydrocannabinol's content was 5. 32% ±0. 17% with k value of 2. The result showed that the method was rapid, sensitive and accurate, and was applicable in the quantitative determination of cannabis plant and resin.%建立了大麻毒品中主要有效成分Δ9-四氢大麻酚的液相色谱分析方法.选择甲醇作为大麻植物或大麻树脂的提取溶剂.采用岛津ODS-SP型(150 mm×4.6 mm,5μm)色谱柱作为定量方法的标准柱,紫外检测器主定量波长为210 nm,辅定量波长为195 nm或220 nm.以水-乙腈为流动相进行梯度洗脱,流速1mL/min,柱温40℃,进样量10 μL.在优化条件下,△9-四氢大麻酚在0.5～ 100.0 mg/L范围内线性良好,外标法及内标法的相关系数(r2)均大于0.99999.方法的检出限(S/N≥3.3)和定量下限(S/N≥ 10)分别为0.12 mg/L和0.40 mg/L.用该方法检测某大麻树脂样本,△9-四氢大麻酚含量的不确定度评定结果为5.32％±0.17％,k=2.实验结果表明,该方法快速、灵敏、准确、可靠,适用于大麻植物及树脂的定量测定.
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