A novel fluorescence enhancing probe for Hg2+,3',6'-bis (diethylamino)-2-((2-hydroxy-4-methoxybenzylidene) amino) spiro [isoindoline-1,9'-xanthene]-3-thione (RBS),was designed and synthesized.Its structure was characterized with IR,elemental analysis and 1 H NMR spectra.The fluorescence intensity of probe solution is very weak,but after combination with Hg2+,its fluorescence intensity increased obviously.Thus,a new method for the determination of Hg2 + was established.It exhibits a dynamic response concentration range for Hg2+ from 5.00 × 10-9 mol/L to 1.10 × 10-6 mol/L with a detection limit of 2.82 × 10-9 mol/L.The method showed advantages of simplicity,high sensitivity,excellent selectivity and wide linear range,and was applied in the determination of Hg2+ in real sample with satisfactory result.%设计合成了一种可逆的Hg2荧光增强型分子探针3′,6′-双(二乙氨基)-2-((2-羟基-4-甲氧基苯亚甲基)氨基)螺[异吲哚-1,9′-氧杂蒽]-3-硫酮(RBS),并利用红外光谱、元素分析、核磁等方法对探针结构进行表征.探针本身荧光很弱,与Hg2结合后荧光显著增强,由此,建立了Hg2+测定的新方法.该方法对Hg2测定的线性范围为5.00×10-9～1.10×10-6 mol/L,检出限为2.82×10-9 mol/L,具有操作简单、灵敏度高、选择性好、线性范围宽等特点.将该方法用于河水及土壤样品中Hg2的加标回收实验,结果满意.
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