苏丹红Ⅰ磁性分子印迹聚合物的制备及其分离分析应用

摘要

以3-(异丁烯酰氧)丙基三甲氧基硅烷修饰的Fe3O4为磁性组分,苏丹红Ⅰ为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,制备了磁性分子印迹聚合物,并用扫描电镜、红外光谱和磁强计对其进行表征.Scatchard分析表明,该磁性印迹聚合物有两类结合位点,最大表观结合量分别为84.59、210.49 μmol/g;磁性印迹聚合物对苏丹红Ⅰ/苏丹红Ⅱ、苏丹红Ⅰ/苏丹红Ⅲ的相对选择系数分别为2.47和2.24,表明该印迹聚合物对苏丹红Ⅰ有较高的选择性和吸附性.将该磁性印迹聚合物应用于辣椒粉提取液中苏丹红I的分离富集,用磁铁将其与溶液快速分离,经高效液相色谱测得其检出限为0.50 μg/g,样品回收率为78％～ 103％,相对标准偏差为2.8％～ 5.8％.%Novel magnetic molecularly imprinted polymers(M-MIPs) were prepared using Sudan Ⅰ as template by molecularly imprinting technique.The morphology,composition and magnetic properties of magnetic nanoparticles were characterized by scanning electron microscope,infrared spectroscopy and vibrating sample magnetometer.The Scatchard analysis showed that there were two kinds of binding sites between the imprinted polymers and the template.The apparent maximum number (Qmax) of the M-MIPs were 84.59 μmol/g and 210.49 μ mol/g,respectively.The relative selectivity coefficient of M-MIPs toward Sudan Ⅰ/Sudan Ⅱ and Sudan Ⅰ/Sudan Ⅲ were 2.47 and 2.24,respectively,which indicated that the M-MIPs exhibited better adsorption properties and selectivity for Sudan Ⅰ.The M-MIPs were applied in the separation of Sudan I in the extracting solution of paprika.The eluant after treating with the M-MIPs was determined by HPLC.The detection limit was 0.50 μg/g.The recoveries of Sudan I ranged from 78％ to 103％ with RSDs of 2.8％-5.8％.