羟基磷灰石表面形貌对人成骨肉瘤细胞生物学性能的影响

摘要

The effect of surface topography of hydroxyapatite (HA) on Human osteosarcoma MG-63 cell lines response was investigated.HA discs with various pore sizes,different pore morphology and distribution were produced by single-axis pressing method.Cell morphology and proliferation were evaluated by scanning electron microscope (SEM) and MTT methods,respectively,and the differentiation potential was assessed by alkaline phosphatase (ALP)activity testing and osteogenic gene expression (RT-PCR).The results showed that the HA discs with macroporous structure (＞200 μm) would promote cell adhesion and proliferation,while osteogenic differentiation of MG-63 cells was facilitated by the HA discs with micropore structure (＜100 μm).Moreover,the differentiation was also influenced by regularity and distribution of the pore.The irregular and alveolate micropore showed a stronger ability of osteogenic induction than smooth and shallow micropore structure.%本文研究了羟基磷灰石(HA)表面形貌对人成骨肉瘤细胞(MG-63)生物学性能的影响.通过单轴压片技术与粒子占位法相结合控制陶瓷表面孔尺度、形态及分布,从而获得具有不同表面孔结构的HA陶瓷材料.将材料与MG-63共培养,通过扫描电子显微镜(SEM),MTT检测法表征材料表面形貌对细胞的黏附和增殖影响,并通过碱性磷酸酶活性(ALP)检测和实时荧光定量(RT-PCR)技术探讨了HA陶瓷材料的表面结构对MG-63成骨分化的诱导作用.结果表明,大孔结构(孔径大于200 μm)更有利于细胞的黏附和增殖,而小孔结构(孔径小于100 μm)能促进细胞的成骨分化.孔形貌和孔分布也能影响细胞的生物功能,相同尺度的孔径,不规则蜂窝状的多级微孔结构比光滑孔壁的浅孔结构更能诱导细胞的成骨分化.