分光光度法测定蒙药耧斗菜中盐酸小檗碱的含量

摘要

Objective:To establish a method for determinating content of berberine hydrochloride in Mongolian medicine Aquilegia viridiflora Pall. Methods:Using ultrasonic method with methanol as the extraction solvent to extract berberine hydrochloride in the samples growed in Manhan mountain. With methanol as blank,the content of berberine hydrochloride was determined by spectrophotometry at the wavelength of 348nm. Results:Berberine hydrochloride concentration in the range of 0. 0020 ~0. 0140 mg/mL and the absorbance showed a good linear relationship,and the average rate of recovery was 96 . 41%( n=6 ) . The content of berberine hydrochloride in Mongolian medicine Loudoucai which from three different regions, Xiaojinggou, Manhan outain, Sanchagou respectively was 6. 259 mg/g , 5. 630 mg/g and 5. 960 mg/g . Conclusion:The method is simple, repeatable and can be used for content determination of berberine hydrochloride in Mongolia medicine.%目的：建立蒙药耧斗菜中盐酸小檗碱的含量测定方法。方法：以甲醇为提取溶剂，采用超声法直接提取样品中的盐酸小檗碱，以蛮罕山产蒙药耧斗菜作为供试品对本实验方法进行考察，以甲醇为空白，在348nm波长处用紫外分光光度法测定盐酸小檗碱的含量。结果：盐酸小檗碱浓度在0.0020~0.0140 mg/mL范围内与吸光度呈良好的线性关系，平均加样回收率为96.77%(n=6)，小井沟、蛮罕山、三岔沟三不同产地蒙药耧斗菜中盐酸小檗碱的含量分别为6.259 mg/g、5.630 mg/g及5.960 mg/g。结论：本法操作简便、重复性好，可用于中蒙药材中盐酸小檗碱的含量测定。