二苯乙烯苷和三七总皂苷配伍对Aβ25-35致PC12细胞损伤影响的研究

摘要

目的 探讨二苯乙烯苷(TSG)和三七总皂苷(PNS)配伍对Aβ25-35诱导的PC12细胞损伤的影响. 方法 PC12细胞采用Aβ25-35诱导建立阿尔茨海默病的损伤模型,被随机分成空白组、模型组、多奈哌齐组(10 μmol/L)、TSG(100 mmol/L)组、PNS组(50 mg/L)、TSG-PNS配伍组(TSG100 mmol/L+PNS50 mg/L). 取细胞上清液测各组的乙酰胆碱酯酶(AchE)及超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量. 结果 与模型组比较,TSG组、PNS组、TSG-PNS配伍组可降低AchE活力、MDA含量及提高SOD活力(P<0.05). 且TSG-PNS配伍组的效应比TSG或PNS单用组效应更好(P<0.01). 结论 TSG和PNS配伍对Aβ25-35诱导的PC12细胞损伤具有明显的保护作用,其作用机制可能与胆碱能系统及氧化应激有关.%Objective To investigate the effect of compatibility of TSG and PNS on PC12 cell injury induced by Aβ25-35. Methods PC12 cell induced by Aβ25-35 was established Alzheimer's disease damage models, and PC12 cells were randomly divided into blank group, model group, Donay Patsy group (10 μmol/L), TSG group (100 mmol/L), PNS group (50 mg/L), TSG-PNS group (TSG 100 mmol/L + PNS 50 mg/L).The acetylcholinesterase (AchE), catalase (SOD) activity and malondialdehyde (MDA) content in supernatant were measured. Results Compared with the model group, the TSG group, PNS group and TSG-PNS group can reduce the vigor of AchE and the content of MDA, and increase the activity of SOD(P<0.05). Moreover, the effect of TSG-PNS group was better than only using TSG or PNS (P<0.01). Conclusion The compatibility of TSG and PNS exhibited a significant protective effect on PC12 cell injury induced by Aβ25-35 and its mechanism may be related to the cholinergic system and oxidative stress.