止痛健骨方对兔膝骨关节炎模型滑膜及软骨修复的影响

摘要

Objective To observe the effect of Zhitong Jiangu decoction on synovial and cartilage repair in rabbit models of knee osteoarthritis. Methods The rabbits were randomly divided into normal control group, model control group, Gucining group, and low, medium and high dose of Zhitong Jiangu groups, six rabbits in each group, after rabbit models of knee osteoarthritis were successfully established. Rabbits of low, middle, high dose of Zhitong Jiangu were given 4.48, 8.96 17.92 g/kg Zhitong Jiangu decoction, respectively. Gucining group was given 0.504 g/kg Gucining capsule, while normal control group and model control group were given 10 mL/kg distilled water for once per day, continuously administration for 4 weeks. Then right knee flexion mobility, synovium thickness and ratio of noncalcified cartilage with calcified, and synovial fluid inflammatory cells were observed and compared. Results After 4 weeks, compared with normal control group, the right knee flexion mobility and ratio of noncalcified cartilage with calcified of the model control group was decreased, while its synovium thickness and synovial fluid inflammatory cells was increased (P<0.05). Compared with model control group, the knee flexion mobility and ratio of noncalcified cartilage with calcified of Zhitong JianGu groups were increased, while their synovium thickness and synovial fluid inflammatory cells were decreased, the diffrences were statistically significant (P <0.05). Conclusion Zhitong Jiangu decoction could inhibit the synovium hyperplasia, thicken noncalcified cartilage, delay cartilage degeneration and increase knee flexion mobility, it may be a mechanism for the treatment of knee osteoarthritis.%目的：观察止痛健骨方对兔膝骨关节炎模型滑膜及软骨修复的影响。方法建立兔膝骨关节模型，将兔随机分为正常对照组，模型对照组，骨刺宁组，止痛健骨低、中、高组共6组，每组6只，止痛健骨低、中、高剂量组分别给予4.48、8.96、17.92 g/kg止痛健骨方灌胃，骨刺宁组给予0.504 g/kg骨刺宁胶囊灌胃，正常对照组、模型对照组给予蒸馏水灌胃，每次10 mL/kg，每日1次，连续给药4周，检测各组兔右膝关节屈曲活动度、关节滑膜厚度及软骨未钙化厚度/钙化厚度，以及膝关节滑膜液炎性细胞，并进行比较。结果实验4周后，模型对照组较正常对照组膝关节屈曲活动度、软骨未钙化厚度/钙化厚度明显减少，关节滑膜厚度、滑膜液炎性细胞明显增加（P＜0.05）。与模型对照组比较，止痛健骨低、中、高剂量组膝关节屈曲活动度、软骨未钙化厚度/钙化厚度均明显增加，关节滑膜厚度、滑膜液炎性细胞减少，差异有统计学意义（P＜0.05）。结论止痛健骨方能抑制滑膜增生，增加非钙化软骨厚度，延缓软骨退变，增加关节屈曲活动度，这可能是其治疗膝骨关节炎的作用机制。