The study investigated the effects of heat shock protein 70(HSP70) antisense oligonucleotide(ASODN) on the proliferation and apoptosis of a human hepatocellular carcinoma cell line(SMMC-7721 cells) in vitro.HSP70 oligonucleotide was transfected into SMMC-7721 cells by the mediation of SofastTM transfection reagent.Inhibition rate of SMMC-7721 cells was determined by using MTT method.Apoptosis rate and cell cycle distribution were measured by flow cytometry.Immunocytochemistry staining was used to observe th...
展开▼
机译:Yield lncreasing and Quality lmproving Effects of Smash-ridging Method ("4453"Effects) and lts Potential in Benefiting the Nation and the People粉垄“4453”增产提质效应及其利民利国发展潜能