宫颈癌中 KAI1／CD82、ILK表达变化及与术后复发转移关系研究

摘要

目的：研究KAI1／CD82和ILK蛋白在宫颈鳞癌（ SCC）组中的表达变化情况，探讨两者与SCC发生发展的相关性。方法收集20例正常宫颈组织、40例CIN组织及40例SCC组织，应用免疫组织化学方法检测KAI1／CD82和ILK蛋白的表达，结合临床病理参数及2年的随访资料，分析两者与SCC的复发转移的关系。结果 KAI1／CD82在正常宫颈组、CIN组和SCC组的表达水平均显著降低（P＜0．05），KAI1／CD82蛋白表达与宫颈癌的分化程度、浸润深度、淋巴结转移及脉管浸润差异密切相关（P＜0．05）；ILK在正常宫颈组、CIN组和SCC组的表达水平均显著升高（P＜0．05），ILK蛋白表达与宫颈癌的浸润深度、淋巴结转移及脉管浸润差异密切相关（P＜0．05）；相关性分析表明，两者表达呈现负相关（r＝－0．4164，P＜0．05）；经Cox比例风险模型，行单因素及多因素分析发现，KAI1／CD82和脉管瘤栓是宫颈癌术后复发的相关因素。结论（1）KAI1／CD82表达下调和ILK表达上调在宫颈癌的发生发展中可能起到一定作用。（2）KAI1／CD82和ILK在宫颈癌中的表达呈负相关，他们共同参与了宫颈癌的发生、发展和转移。（3）KAI1／CD82可以作为预测宫颈癌根治术后复发转移潜能的参考指标。（4）KAI1／CD82和淋巴结转移是宫颈癌术后复发的独立因素。%Objective To study the expression of KAI1/CD82 and ILK in cervical squamous cell carcinoma (SCC),and to explore the relationship between the two indicators and the development of SCC.Methods Retrospective analysis was con-ducted in terms of the clinical and pathological data and two years'follow-up data of 20 cases of normal cervical tissues,40 ca-ses of cervical intraepithelial neoplasia( CIN) and 40 cases of SCC.Expressions of KAI1/CD82 and ILK in those samples were measured with immunohistochemical method and the relationship between the two indicators and the recurrence and metastasis of SCC were analyzed.Results The protein expression levels of KAI1/CD82 in the normal cervical tissues,CIN and SCC were significantly reduced(P<0.05).KAI1/CD82 expression was closely related to the differentiation,depth of invasion,lymph node metastasis and vascular invasion of SCC(P<0.05),while the protein expression levels of ILK in the three groups were significantly increased(P<0.05).The protein expression levels of ILK were related to the depth of invasion,lymph node me-tastasis and vascular invasion of SCC(P<0.05).The protein expression levels of KAI1/CD82 were negatively correlated to ILK(r=-0.4164,P<0.05);Cox proportional hazards univariate and multivariate analysis showed the protein expression lev-els of KAI1/CD82 and vascular tumor thrombus were closely related to the recurrence of cervical cancer.Conclusion Down-regulating of the expression of KAI1/CD82 and upregulating of ILK have an effect on the development of cervical carcinoma. The protein expression levels of KAI1/CD82 are negatively correlated to ILK,and they are related to the development and me-tastasis of cervical carcinoma.KAI1/CD82 and ILK can become the postoperative indicators of recurrence and metastasis of cervical carcinoma.Analysis shows that KAI1/CD82 and lymph node metastasis accelerates the recurrence of cervical cancer.