糖尿病大鼠血糖波动对肝脏功能及超微结构的影响

摘要

目的 建立糖尿病(diabetes mellitus,DM)大鼠血糖渡动模型,观察血糖波动对DM大鼠肝脏损伤的作用.方法 SD 大鼠24只均分为3组,A组为正常对照组,B组为稳定高血糖组,C组为波动血糖组,B组、C组大鼠均一次性腹腔内注射链脲佐菌素(STZ)65 mg/kg,建立DM模型.C组每天错时给予胰岛素和50%葡萄糖造成血糖波动模型.12周后测定血清谷丙转氨酶(AST)、谷草转氨酶(ALT)和肝脏指数,观察肝脏超微结构改变.结果与A组比较,B和C组肝脏指数明显升高(分别为P<0.05和P<0.01),血清AST和ALT升高(均P<0.01),超微结构明显改变.C组肝脏指教高于B组(P<0.05),C组AST、ALT明显高于B组(均P<0.01),超微结构改变较B组加重.结论 糖尿病大鼠12周时肝脏出现明显损伤,且血糖波动能加重其损伤程度.%Objective To establish the animal model of diabetic rats with fluctuant high blood glucose and observe the effects of fluctuant high blood glucose on liver in diabetic rats. Methods Twenty-four SD rats were divided into 3 groups, including control group (group A), stable high blood glucose group (group B) and fluctuant high blood glucose group (group C). Diabetes mellitus model was induced by an intraperitoneal injection of STZ at a dose of 65 mg/kg body weight in group B and group C. Fluctuant high blood glucose model in group C was induced by intraperitoneal injection of insulin and glucose at different time points every day. Liver index, the levels of serum AST and ALT were determined and hepatic ultrastructure was observed in all groups after 12 weeks. Results Liver index and the levels of serum AST and ALT in group B and group C were obviously increased (P＜0.05 or P＜0.01) in comparison with group A. Alterations of ultrastructure was more obvious in group B and group C than that in group A. Liver index and the levels of serum AST and ALT were greater in group C than those in group B (P＜0.01 or P＜0.05). Uhrastructure damage was more severe in group C than that in group B. Conclusion Obviously injury in liver in diabetic rats occurs in 12 weeks. Fluctuant high blood glucose aggravates the injury of the liver in diabetic rats.