小麦新品种百农矮抗58及其亲本矮秆基因的检测

摘要

为探索百农矮抗58小麦所含的矮秆基因及其来源,应用赤霉素反应和分子标记检测了百农矮抗58及其亲本周麦11、豫麦49号、郑州8960的矮秆基因.结果表明:郑州8960为赤霉素敏感型,其他3个品种对赤霉素不敏感；分子标记检测显示,矮抗58、豫麦49号、郑州8960携带Rht -D1b基因,周麦11携带Rht -B1b基因,4个品种均没有扩增出Rht8基因的192 bp标准带.%In order to explore the source of dwarfing genes in wheat variety AK58.AK58 and its parents Zhoumai 11, Yumai 49 and Zhengzhou 8960 were analyzed by means of gibberellic acid (GA3) reaction and STS markers. The results indicated that Zhengzhou 8960 was sensitive to GA3 and other cultivars were insensitive. Molecular marker confirmed that Rht-Dlb was detected in AK58,Yumai 49 and Zhengzhou 8960,Rht-Blb was present in Zhoumai ll,while.no Rht8 gene was found in above 4 varieties.