小麦抗白粉病基因 Pm13的 SSR 标记筛选

摘要

为筛选与抗白粉病基因 Pm13紧密连锁的分子标记，以携带 Pm13的抗病品系中大01与感病品系金光588为亲本进行杂交，获得 F1、F2分离群体和 F2：3家系，利用分离群体分组分析法（BSA）进行 SSR 标记分析。结果表明，中大01携带的 Pm13基因位于3BS 染色体，5个 SSR 标记BE398268、wmc674、cfa2226、gwm533．1和 BE471274与 Pm13连锁，遗传距离分别为0·5、0·8、1·6、13·2、52·1 cM，其中紧密连锁的标记 BE398268、wmc674和 cfa2226可用于该基因的分子标记辅助选择。%To detect molecular markers closely linked to wheat powdery mildew resistance gene Pm13 ,bulked segregant analysis (BSA)was used to identify microsatellite (simple sequence repeats,SSR)markers in the F2 population from the cross Zhongda 01(resistant)×Jinguang 588 (susceptible).The results indicated that Pm13 in Zhongda 01 was located in the 3BS region, linked to five SSR markers BE398268,wmc674,cfa2226,gwm533·1 and BE471274 with the genetic distances of 0·5 cM,0·8 cM,1·6 cM,13·2 cM and 52·1 cM respectively.The molecular markers BE398268,wmc674,and cfa2226 closely linked to Pm13 can be used in maker-assisted selection(MAS)in wheat breeding program for powdery mildew resistance.