河南地区猪源性大肠杆菌血清型鉴定与耐药性调查

摘要

为了解河南省规模化猪场大肠杆菌的流行概况，无菌采集疑似大肠杆菌感染的病死猪病料185份，经常规细菌培养、生化试验及致病性试验，分离到86株大肠杆菌；对分离菌株进行血清型鉴定，确定了67株大肠杆菌血清型，分属15个血清型，主要以 O8、O9、O161、O107为主。测定了已鉴定血清型的67株大肠杆菌对15种常用抗菌药物的耐药性，结果显示，对阿米卡星、黏菌素、头孢曲松钠、头孢噻呋敏感率高的菌株较多；对环丙沙星、多西环素、阿莫西林、四环素、磺胺甲恶唑耐药的菌株达80％以上，对四环素和磺胺甲恶唑耐药的菌株比例最高，分别为88.1％、89.6％；67株菌株均表现不同程度的多重耐药，每种菌株至少对3种受试药物耐药，53.7％的菌株对9种以上药物耐药。表明河南地区的猪源大肠杆菌耐药现象严重，耐药谱广。%In order to understand the Escherichia coli epidemiological situation in scale pig farms of Henan province,185 strains were collected from silk and death swine which were suspected Escherichia coli infection.Eighty-six strains of Escherichia coli were isolated from those samples after conventional bacterial culture,serotype identification and lethal test,and serotype of 6 7 strains were identified.The identified bacteria belonged to 15 kinds of serotype distributed,which were mainly O8,O9,O161,O107. The 6 7 identified strains drug sensitivity were tested to 1 5 kinds of drugs.The results showed that most of the identified bacteria were sensitive to Amikacin,Colistin,Cefatriaxone,Ceftiofur.Eighty percent of identified bacteria was resistant to Ciprofloxacin,doxycycline,amoxicillin,tetracycline,sulfamethoxazole. These identified strains showed the highest resistant to tetracycline and sulfamethoxazole,which were 88.1% and 89.6%,respectively.The 67 identified strains were all multi-drug resistant in some degree. Isolated strains could resist at least 3 kinds of commonly used antibiotics.Fifity-three point seven percent strains were resistant to 9 kinds of drug.This study showed that the Escherichia coli resistant situation was serious and the resistance showed a broad spectrum in Henan province.